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系统工程提高酵母酵母中的烯醇生产

Qi Gao^1,2, Herong Wang^1,2, Mengying Shan^1,2

¹Frontier Science Center for Synthetic Biology and State Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Journal of agricultural and food chemistry

|June 6, 2025

概括

在这项研究中,他们利用酵母来制造一种有价值的香料化合物 - - citronellol. 基因改造显著提高了产量,实现了可持续生物合成的创纪录10.556g/L标位.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

罗醇是一种非循环单醇,在食品和农业中广泛使用.
微生物细胞工厂为龙生产提供了一个可持续的途径.
关键的瓶包括前体供应 (杰拉尼尔酸盐,NADPH) 和单烯细胞毒性.

研究的目的:

通过代谢工程来增强*Saccharomyces cerevisiae*中的烯醇生产.
为了克服前体限制和细胞毒性挑战.
建立一个基于高位酵母的生产系统,以生产 citronellol.

主要方法:

整合了美酸盐路径和氧体局部基因的额外副本.
过度表达的酸通路基因 (TAL1,TKL1) 来促进NADPH供应.
选的内源转运器和集成的PDR1用于改善出口.

主要成果:

通过基因整合途径实现了罗醇产量的1.5倍增加.
通过酸通路的基因过度表达,增加了16%的罗醇产量.
通过选输送器和集成PDR1.1,达到3.38g/L的标位.
在100升料批发发酵中达到创纪录的10.556g/L青醇标位.

结论:

酵母机架细胞的系统修改显著增加了罗醇标位.
开发的策略为其他单烯的生物合成提供了基础.
这项工作展示了一种高效且可扩展的酵母平台,用于生产烯醇.

关键词:

在MVA路径上.提供NADPH供应的NADPH.这种植物是Saccharomyces cerevisiae.在这种情况下,Citronellolol是最好的.过氧酶体的局部化运输工程运输工程运输工程

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