Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

4.9K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.9K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

3.0K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.0K
Antimicrobial Proteins01:23

Antimicrobial Proteins

6.5K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
6.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Telomere heterochromatin-mediated compartmentalization: Where ALT begins.

Trends in cell biology·2026
Same author

Using generative AI to transform peptide hits into small molecule leads.

Beilstein journal of organic chemistry·2026
Same author

Identification and characterization of WpDestabilase, a novel destabilase from the salivary glands of Whitmania pigra.

Protein expression and purification·2026
Same author

Diallyl trisulfide activates Nrf2 by promoting S-sulfhydration of Keap1 to attenuate acute hypobaric hypoxia-induced lung injury.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Transcriptomic analyses of labial glands and gut tissue from two wax moths, Achroia grisella and Galleria mellonella.

G3 (Bethesda, Md.)·2026
Same author

Correction: Targeted Therapy of TERT-Rearranged Neuroblastoma with BET Bromodomain Inhibitor and Proteasome Inhibitor Combination Therapy.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026

相关实验视频

Updated: Sep 19, 2025

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

11.4K

强大的循环抑制剂破坏了FANCM-RMI相互作用.

Lisa J Alcock1, Tianyi Gao1, Rohan Bythell-Douglas2

  • 1School of Chemistry, The University of Sydney, Camperdown, NSW 2145, Australia.

Journal of medicinal chemistry
|June 6, 2025
PubMed
概括

研究人员发现了首个针对FANCM-RMI蛋白相互作用的化学抑制剂,这对于替代性延长端粒 (ALT) 途径癌症中基因组稳定性至关重要. 这些强大的循环抑制剂为癌症治疗开发提供了新的途径.

更多相关视频

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

12.6K
Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.0K

相关实验视频

Last Updated: Sep 19, 2025

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

11.4K
Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

12.6K
Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.0K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 癌症研究 癌症研究

背景情况:

  • FANCM-RMI蛋白与蛋白相互作用对于保持基因组稳定性至关重要.
  • 这种相互作用在使用替代延长端粒 (ALT) 途径生存的癌症中至关重要.

研究的目的:

  • 确定和描述FANCM-RMI相互作用的第一个化学抑制剂.
  • 探索这些抑制剂在开发新的癌症疗法方面的潜力.

主要方法:

  • 使用mRNA显示器对循环的查以识别抑制剂.
  • 抑制剂结合亲和力 (KD) 和破坏性强度 (IC50) 的表征.
  • 进行X射线结晶学,氨酸扫描和共免疫沉研究,以阐明结合模式和细胞内活性.

主要成果:

  • 发现了强有力的循环抑制剂,可以利用RMI1/2结合口袋,具有纳米分子亲和力 (KD = 2-10 nM).
  • 抑制剂显著破坏了FANCM-RMI相互作用 (IC50 = 54-104 nM).
  • 结构研究揭示了新的结合模式,基于细胞的测试证实了本地相互作用的破坏.

结论:

  • 这些循环是FANCM-RMI相互作用的第一个验证的抑制剂.
  • 它们作为研究基因组稳定机制的有价值的化学工具.
  • 它们代表了开发针对ALT驱动癌症的治疗方法的有希望的起点.