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在Munjal绵羊中,与热耐受性特征相关的HSP70基因的表达和SNP概况

Sunil Kumar¹, Ankit Magotra², Narender Kumar³

¹Department of Livestock Farm Complex, LUVAS, Hisar, 125004, Haryana, India; Department of Animal Genetics and Breeding, LUVAS, Hisar, 125004, Haryana, India.

Journal of thermal biology

|June 6, 2025

概括

蒙贾尔羊中发现了热冲击蛋白70 (HSP70) 基因的多态性,表达水平因季节,年龄和耐热性而异. HSP70 作为家畜耐热性的潜在标志物.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
畜牧科学畜牧科学畜牧科学

背景情况:

热应激对牲畜生产率构成重大威胁.
热冲击蛋白 (HSP),如HSP70,在细胞对压力的反应中起着至关重要的作用.
识别耐热性遗传标记对于动物适应气候变化至关重要.

研究的目的:

为了研究蒙贾尔绵羊中热冲击蛋白70 (HSP70) 基因的多态性.
分析HSP70的表达特征,与季节性变化,年龄和耐热性相关.
评估HSP70作为一种潜在的生物标志物,用于羊的耐热性.

主要方法:

从Munjal羊的血液样本中提取DNA.
聚合酶连锁反应 (PCR) 和单核酸多态性 (SNP) 排序对HSP70基因进行分析.
与酶相关的免疫吸收试验 (ELISA) 用于在不同季节和群体中分析HSP70表达.
包括t测试在内的统计分析,用于比较基因表达水平.

主要成果:

在绵羊的HSP70基因中发现了单个核酸多态 (c.459的A到G突变),导致AG和AA基因型.
在夏季和冬季季节之间,HSP70基因表达有显著的变化.
成年羊的HSP70表达明显高于羔羊.
耐热 (HST) 绵羊与热敏感 (HSS) 绵羊相比,显示出明显不同的HSP70表达.

结论:

这项研究在Munjal羊的HSP70基因中发现了一种特定的SNP.
蒙贾尔羊的HSP70表达受季节,年龄和耐热状态的影响.
HSP70具有作为选择耐热羊品种的分子标记物的潜力,以改善在恶劣气候条件下牲畜的弹性和生产力.

关键词:

适应适应适应热应激是一种热应激.耐热系数耐热系数耐热系数蒙贾尔羊是蒙贾尔羊的一种羊.这是直肠温度的温度.

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相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...