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通过间隙结的细胞类型特异性核酸共享影响了Drosophila中对复制应激的敏感性

Benjamin Boumard¹, Gwenn Le Meur¹, Laïla Aboutine¹

¹Institut Curie, PSL Research University, Sorbonne University, CNRS UMR 3215, INSERM U934, Stem Cells and Tissue Homeostasis Group, Paris, France.

Developmental cell

|June 6, 2025

概括

细胞可以通过差距连接非自主地共享必需的脱氧核酸 (dNTP),以缓冲耗尽. 这就解释了为什么一些细胞,比如Drosophila ISCs,对dNTP损失敏感,而其他细胞,比如翅膀细胞,则不如此.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
分子生物学分子生物学

背景情况:

细胞增殖需要大量的脱氧核化物 (dNTP) 来进行DNA合成.
细胞dNTP水平通常由内在的de novo合成和救援途径来调节.
细胞之间非自主提供代谢物是一种潜在的,但尚未研究的,维持组织平衡的机制.

研究的目的:

研究细胞间通信在维持脱氧核酸 (dNTP) 池恒温的作用.
为了确定细胞是否可以在体内从邻近的细胞中接收dNTP.
了解各种细胞类型对核酸枯竭的不同敏感性.

主要方法:

采用Drosophila上皮组织,包括成年肠干细胞 (ISC) 和翅膀前代细胞,作为模型系统.
研究了间隙结在细胞间代谢物交换中的作用.
评估细胞对核酸枯竭和复制压力的敏感性.

主要成果:

证明核酸可以通过间隙结连接非自主地供应给细胞.
翅膀前代细胞,拥有间隙结,缓冲对核酸枯竭诱导的复制压力.
成人ISC缺乏间隙结,因无法从邻居那里接收dNTP而对核酸耗尽非常敏感.

结论:

间隙连接依赖的细胞间共享在体内显著促进脱氧核酸 (dNTP) 池恒温.
间隙结的差异表达和透性影响细胞对细胞内dNTP水平波动的敏感性.
这突显出一种新的代谢合作机制,用于维持组织健康和功能.

关键词:

差距连接点差距连接点差距连接点核酸恒常化是核酸的恒常化.复制压力是复制的压力.干细胞是干细胞的组成部分.组织恒温组织恒温.

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