精细的映射和功能验证揭示了两个不完整的主导基因,这些基因控制了Gossypium hirsutum中光周期敏感的开花
Langjing Hua1, Chao Xu1, Xiao Liu1
1College of Advanced Agricultural Sciences, Zhejiang A&F University, Linan, Hangzhou, 311300, Zhejiang, China.
概括
两种基因GHAGL8和GhJAZ1被确定为高地棉花开花时间的关键调节者. 这些基因被映射到D03和D08染色体上,为Gossypium hirsutum的光周期适应提供了洞察力.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 农业学是一种农业学.
背景情况:
- 高地棉花 (Gossypium hirsutum) 的开花时间对作物产量至关重要,并且与光周期敏感性密切相关.
- 第一个果实分支 (NFFB) 的节点位置是光周期敏感开花的关键指标.
研究的目的:
- 为了识别和绘制基因控制光周期敏感的花期在Gossypium hirsutum.
- 研究候选基因GhaGL8和GhJAZ1在调节NFFB和开花时间方面的作用.
主要方法:
- 利用F2绘制人口从一个晚期开花的陆地品种 (Palmeri 37) 和一个现代品种 (CCRI74) 之间的交叉.
- 进行了精细映射,序列分析,亚细胞定位,基因表达分析和病毒诱导的基因沉默 (VIGS).
主要成果:
- 将NFFB的两个主要定量特征位置 (QTL) 映射到D03和D08染色体上.
- 确定了AGAMOUS-LIKE8 (GhAGL8) 和JASMONATE ZIM-DOMAIN1 (GhJAZ1) 作为候选基因.
- GhAGL8和GhJAZ1在父系之间表达差异,GhaGL8的VIGS延迟了NFFB,而GhJAZ1表现出潜在的功能变异.
结论:
- GhAGL8和GhJAZ1是高地棉花中光周期敏感的开花时间的新型关键调节者.
- 在GhAGL8促进体和氨基酸插入GhJAZ1的哈普洛型变异与开花时间的差异有关.
- 帕尔梅里37是研究棉花光周期适应机制的宝贵生殖质资源.
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