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基于LC-MS/MS的同时量化乙卡尼丁,乙酸和酸:探索人类血中的潜在生物标志物

Baiba Gukalova^1,2, Kristaps Krims-Davis^3,4, Eduards Sevostjanovs³

¹Latvian Institute of Organic Synthesis, Aizkraukles Str 21, Riga, LV1006, Latvia. baiba.gukalova@osi.lv.

Analytical and bioanalytical chemistry

|June 6, 2025

概括

来自多不和脂肪酸 (PUFA) 的新型乙卡尼因作为PUFA摄入量的敏感标记物具有前景. 这项研究开发了一种快速的LC-MS/MS方法来量化这些新型标记物,帮助omega-3补充剂研究.

科学领域:

生物化学生物化学
分析化学分析化学
营养科学营养科学

背景情况:

乙卡尼丁是脂肪酸代谢和线粒体功能的已知生物标志物.
多不和脂肪酸 (PUFA),特别是欧米茄-3脂肪酸,在健康中起着至关重要的作用.
从PUFA衍生出来的乙卡尼丁以前没有被研究过,这限制了它们作为生物标记物的使用.

研究的目的:

开发和验证一种新的LC-MS/MS方法,用于同时量化EPA,DHA及其相应的乙卡尼丁 (EPAC,DHAC).
在临床研究中评估PUFA衍生性乙卡尼丁作为PUFA摄入量的敏感标记物的有用性.

主要方法:

开发了一种快速 (5分钟) 的LC-MS/MS方法来量化人体血中的EPA,DHA,EPAC和DHAC.
采用蛋白质沉用于样本提取和背景减去用于矩阵效应正常化.
在各种存储和处理条件下验证了方法的精度,准确性和分析物的稳定性.

主要成果:

经过验证的LC-MS/MS方法证明了高精度 (<10%CV) 和精度 (<10%偏差) 的低量化极限 (EPA/DHA 2μM,EPAC/DHAC 2nM).
在短期存储 (6小时室温/冷) 和冷-解周期中证实了分析物的稳定性,但在长期存储中没有证实.
一项试点研究显示,在补充PUFA后,与EPA和DHA相比,血EPAC和DHAC的相对增加更高.

结论:

开发的LC-MS/MS方法简单,快速,具有成本效益,适合在临床环境中进行高吞吐量分析.
由PUFA衍生的乙卡尼丁 (EPAC,DHAC) 是PUFA摄入的潜在的新型和敏感生物标志物.
这些发现支持在监测omega-3补充剂和生活方式干预方面使用乙卡尼丁.

关键词:

乙卡尼丁酸盐是一种达科萨赫克萨埃诺酸是什么?达科萨赫克萨埃诺伊尔-L-卡尼丁是什么?埃可萨丁烯酸是什么埃可萨丁烯酸是什么埃可萨丁乙烯-L-卡尼丁是什么在 LCMS/MS 中使用.

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