妊娠期和2型糖尿病对胎儿内皮细胞miRNA表达的影响
1Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Regenerative Medicine Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
怀孕期间的孕产妇高血糖会改变胎儿的微RNA (miRNA) 表达,可能导致子宫内皮功能障碍和心血管疾病 (CVD) 风险. 在糖尿病患者中观察到特定的miRNA和FGF11mRNA变化.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 心血管科学 心血管科学
背景情况:
- 胎儿暴露于高血糖症与通过微RNAs (miRNAs) 的表观遗传变化有关.
- 这些变化可能导致内皮功能障碍,并增加后代患心血管疾病 (CVD) 的风险.
- 调查针对子宫内高血糖反应的特定miRNA变化至关重要.
研究的目的:
- 为了研究28个胎儿内皮小RNA的高血糖引起的变化.
- 确定改变miRNA表达,内皮功能障碍和心血管疾病风险之间的关联.
主要方法:
- 使用TaqMan miRNA数组和qPCR. 的不同表达的miRNA的量化.
- 从怀孕期糖尿病 (GDM) 和2型糖尿病 (T2D) 的妇女的人类静脉内皮细胞 (HUVEC) 中分析miRNA表达.
主要成果:
- 在GDM-HUVEC中减少了miR-140-3p,miR-1307-5p,miR-342-3p和miR-16-5p的表达.
- 降低了miR-126-3p和miR-27a-5p,在T2D-HUVEC中增加了miR-27b-3p.
- 在GDM和T2D-HUVEC中均有上调的miR-29b-3p;在GDM-HUVEC中下调的FGF11mRNA (miR-342-3p目标).
结论:
- 改变的miRNA表达,包括miR-342-3p及其标FGF11mRNA,可能会有助于内皮功能障碍.
- 这些变化发生在糖尿病的HUVEC中,即使在几次通过后在正常的葡萄糖条件下.
- 研究结果表明,母亲糖尿病,胎儿miRNA调节和后代心血管健康之间存在分子联系.
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