共同基因和通路的in-silico识别和实验验证,以破译COPD和MASLD之间的分子联系
Anupama Dubey1, Praveen Kumar2, Tahseen Khan3
1Special Center for Systems Medicine, Jawaharlal Nehru University, New Delhi, 110067, India; Special Center for Molecular Medicine, Jawaharlal Nehru University, New Delhi, 110067, India.
Computers in biology and medicine
|June 7, 2025
概括
研究人员确定了12个常见基因和5个枢纽基因,这些基因将慢性阻塞性肺病 (COPD) 与代谢功能障碍相关的脂肪性肝病 (MASLD) 联系起来. 一种针对这些通路的药物显示出对这两种疾病的治疗潜力.
科学领域:
- 生物医学科学 生物医学科学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
背景情况:
- 慢性阻塞性肺病 (COPD) 和与代谢功能障碍相关的脂肪性肝病 (MASLD) 是复杂的疾病,具有共同的全身炎症和氧化还原失衡.
- 对于COPD和MASLD病原体之间的明确分子联系仍然是难以捉摸的.
研究的目的:
- 通过生物信息学方法阐明共同的分子机制,并确定COPD和MASLD之间的共享基因和通路.
- 预测两种疾病的潜在治疗点和候选药物.
主要方法:
- 利用生物信息学工具 (Enrichr,String,Cytoscape) 和公共数据库 (基因表达总汇) 进行网络和途径分析.
- 识别了使用DSigDB.com的常见基因,枢纽基因和预测候选药物.
- 在疾病模型中通过RT-qPCR和COX-2抑制剂 (NS-398) 的治疗评估验证了in-silico发现.
主要成果:
- 确定了12个共同的基因 (例如CXCL8,MMP9,IL1β,CCL2) 和5个中心基因 (例如CCL2,CXCL8) 在COPD和MASLD之间共享.
- 揭示了与两种疾病相关的共享信号通路和基因本体学术语.
- 证明COX-2抑制剂NS-398在COPD和MASLD模型中显著抑制上调基因的表达.
结论:
- 关键的共同和枢纽基因与COPD和MASLD的共享病理生理学有关.
- 已确定的分子标和途径为这两种疾病提供了新的治疗策略的潜力.
- 针对共享的炎症途径,例如通过COX-2抑制,可能对COPD和MASLD患者具有治疗前景.
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