来自无症状死牙的益生菌细菌可以调节微生物群的平衡
Arya Adiningrat1, Ikhsan Maulana2, Ahmad Ghitha Fadhlurrahman2
1Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, Universitas Muhammadiyah Yogyakarta, Indonesia; Molecular Medicine and Therapy Laboratory, Research and Innovation Centre, Universitas Muhammadiyah Yogyakarta, Jl. Brawijaya, Geblagan, Tamantirto, Kec. Kasihan, Kabupaten Bantul, Daerah Istimewa, 55183, Yogyakarta, Indonesia.
Microbial pathogenesis
|June 7, 2025
概括
一种根运河细菌Lacticaseibacillus paracasei被分离出来,它的无细胞超体显示出潜在的后生物效应. 这种超级生物破坏了Streptococcus mutans和Enterococcus faecalis的生物膜,有助于微生物组的平衡.
科学领域:
- 微生物学 微生物学
- 牙周内科医院 牙周内科医院 牙周内科医院
- 生物技术是生物技术.
背景情况:
- 慢性无症状的死根通道是复杂微生物群落的所在地.
- 保持根管微生物组的稳态对于治疗成功至关重要.
- 特定细菌及其产品在这种环境中的作用尚未完全理解.
研究的目的:
- 为了识别和隔离从慢性无症状死根根管中占主导地位的细菌.
- 研究孤立细菌的体外后生物潜力,以维持根管微生物群的恒常性.
- 评估细菌无细胞超眠体 (CFS) 对病原性细菌的抗菌膜作用.
主要方法:
- 甲基因组分析和单个殖民地隔离用于细菌识别.
- 培养Lacticaseibacillus paracasei和制备无细胞超 (CFS) 的方法.
- 在体外对Streptococcus mutans和Enterococcus faecalis进行生物膜分析,使用水晶紫色染色和显微镜 (SEM).
主要成果:
- 主导的分离细菌被确定为Lacticaseibacillus paracasei.
- L. paracasei产生了与Enterococcus faecalis.相似的生物膜质量.
- L. paracasei的CFS对S. mutans和E. faecalis生物膜表现出显著的破坏性影响.
结论:
- 从死根道中分离出来的Lacticaseibacillus paracasei会产生后生物产物.
- 这些后生物有效地破坏了关键口腔病原体S. mutans和E. faecalis的生物膜完整性.
- 这表明L. paracasei衍生后生物在治疗根管感染方面具有潜在的治疗作用.
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