使用热敏聚合物-盐水性双相系统与聚合物回收的聚合物回收,回收氨酸
Nagaraju Marimuthu1, Yin Hui Chow2, Phei Er Kee3
1School of Engineering, Faculty of Innovation and Technology, Taylor's University Lakeside Campus, No. 1, Jalan Taylor's, 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia.
Journal of bioscience and bioengineering
|June 7, 2025
概括
使用热敏聚合物盐水性双相系统 (ABS) 改善了微生物氨酸 (HA) 恢复. 这种环保方法提供了高产量和纯度,可回收聚合物提高了可扩展生产的经济可行性.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 分离科学 分离科学
背景情况:
- 与动物提取相比,微生物发酵是生产氨酸 (HA) 的可持续替代方案.
- 微生物HA的净化对于化品应用至关重要,需要高效且可扩展的分离技术.
研究的目的:
- 调查热敏乙烯氧化物-氧化物 (EOPO) -盐水性双相系统 (ABS) 的疗效,以恢复微生物HA.
- 为了优化ABS参数,包括EOPO分子量,盐类型,相组合和原油提取物度.
- 评估EOPO的可回收性及其对HA回收效率和产品完整性的影响.
主要方法:
- 开发和优化乙烯氧化物-氧化物 (EOPO) -盐水性双相系统 (ABS) 用于氨酸 (HA) 分离.
- 对影响HA分区的参数进行系统研究,包括EOPO分子量 (MW),盐类型,相组合和原油提取物度.
- 使用热分离对EOPO可回收性进行调查,并在回收系统中评估HA回收率.
- 使用富里埃变换红外光谱法 (FTIR) 验证复原后的HA结构完整性.
主要成果:
- 在pH值8.2的14% (w/w) EOPO2500,12% (w/w) 酸和15% (w/w) 粗提取物下,获得了最佳的HA回收,产生了11.41和93.42%的回收分区系数.
- 通过热分离回收了70%以上的EOPO2500,第一轮回收循环保持了HA回收率超过80%的水平.
- 福利埃变换红外光谱学证实,回收的HA保留了其原生结构.
结论:
- 热敏EOPO盐ABS是一种高产量的微生物氨酸回收的有效和环保方法.
- 回收EOPO聚合物显著提高了HA生产过程的经济可行性和环境可持续性.
- 这种优化的ABS方法为工业氨酸生产提供了一个可扩展和更绿色的替代方案,同时保持产品质量.
相关概念视频
04:01Factors Affecting Solubility
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide:
03:11Precipitation of Ions
Predicting Precipitation
The equation that describes the equilibrium between solid calcium carbonate and its solvated ions is:
The equation that describes the equilibrium between solid calcium carbonate and its solvated ions is:
01:09Colloidal precipitates
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
01:17Precipitation and Co-precipitation
Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
01:06Coagulation
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
01:17Ion Exchange
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...