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Pseudocydonia sinensis 几乎没有缺口的基因组组件揭示了独特的烯胺路径

Yangxin Zhang¹, Shuangyu Zhang^2,3, Wenmeng Guo¹

¹State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.

Plant biotechnology journal

|June 8, 2025

概括

我们向您展示了中国 (Pseudocydonia sinensis) 的高质量基因组组,揭示了基因扩展和关键调节者,如PsMYBODO1. 该资源有助于了解其药用特性和未来的育种工作.

科学领域:

植物基因组学植物基因组学
进行比较的基因组学.
代谢学代谢学代谢学

背景情况:

木 (Pseudocydonia sinensis) 在植物学上很重要,但缺乏基因组资源.
它属于rosaceae家族,与果和梨有着密切的关系.
有限的基因组数据阻碍了对其药用特性的研究.

研究的目的:

为了生成一个高质量的,染色体规模,哈普洛型解析的基因组组合P. sinensis.
确定与二次代谢物生物合成相关的基因家族和代谢途径.
为了发现这些途径的关键遗传调节者,以潜在的育种应用.

主要方法:

使用先进的测序技术进行染色体规模的基因组组装.
与相关物种 (例如Malus,Pyrus) 的比较基因组分析.
基因家族扩展分析,代谢组概况分析和转录组分析.
功能性测试用于验证转录因子作用 (例如,PsMYBODO1).

主要成果:

一个高质量的基因组组 (581.29 Mb, 17 个染色体) 具有很高的完整性 (BUSCO, QV 分数).
1603个扩展基因家族的鉴定,特别是那些参与黄类和类生物合成的基因家族.
发现PsMYBODO1作为一个关键的调节器,激活在烯胺路径中的基因.

结论:

的基因组组合是功能基因组学的宝贵资源.
基因重复和扩张有助于其丰富的二次代谢物概况.
了解像PsMYBODO1这样的调节者可以指导育种以增强药用特征.

关键词:

中国的青花.这种植物名为Pseudocydonia sinensis.在 de novo 组装.基因组注释基因组注释二次代谢产物二次代谢产物

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