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来自奶牛场环境的大肠杆菌中抗微生物耐药性的分子特征

Namita Narwal¹, Monica Rose Amarlapudi¹, Ravikant V Vinchurkar¹

¹Molecular Biology Unit, Dairy Microbiology Division, ICAR - National Dairy Research Institute, Karnal, Haryana, 132001, India.

International journal of hygiene and environmental health

|June 8, 2025

概括

乳牛场大肠杆菌的抗菌耐药性很普遍,由多种耐药性基因和移动遗传元素驱动. 持续的基因组监测对于控制乳制品环境中的耐药性传播至关重要.

科学领域:

兽医微生物学兽医微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

细菌中的抗菌素耐药性 (AMR) 对公众健康构成重大威胁.
乳制品农场是抗药性细菌出现和传播的潜在储.
大肠杆菌 (E. coli) 是监测牲畜抗菌素耐药性的关键指标物种.

研究的目的:

调查从奶牛场分离的大肠杆菌菌株中抗菌素耐药性的流行情况.
确定与抗菌素耐药性相关的遗传机制和移动遗传元素 (MGE).
评估抗药性从乳制品环境传播的可能性.

主要方法:

使用选择性培养和PCR从奶牛场样本中分离和识别大肠杆菌.
抗微生物药物耐药性模式的表型特征.
基因型分析以检测特定的耐药基因和MGE (等离子体,转子体,整体体).

主要成果:

观察到对安皮西林,皮佩拉西林,四环素和β-乳酸盐的耐药性高患病率.
显著比例的多药耐药 (MDR) 和ESBL阳性大肠杆菌分离物.
识别不同的AMR基因 (例如,blaCTX-M-1,tetA,tetB,qnrB,gyrA突变) 和MGE (IS26,IncFIB等离子体,TN3转子体).

结论:

乳牛携带大肠杆菌,具有高度多样化的抗菌素耐药性基因.
移动遗传元素在AMR基因的传播中起着至关重要的作用.
持续对乳制品环境的基因组监测对于缓解AMR传播至关重要.

关键词:

抗微生物耐药性抗微生物耐药性奶牛养殖场奶牛养殖场这就是ESBL.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.在MDR中使用MDR.

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