相关实验视频

Updated: Jun 12, 2025

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

调整尺寸的PEGylated NIR-II光探头用于高对比度植物成像和TMV检测

Fang Zhao^1,2, Yu-Ling Xu², Meng-Jiao Lu¹

¹State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, China.

Journal of nanobiotechnology

|June 8, 2025

概括

研究人员设计了各种尺寸的近红外II (NIR-II) 光探针,用于植物生物成像. 最小的探头 (SYH3) 显示了优异的叶脉成像和植物早期病毒感染检测.

科学领域:

植物科学植物科学
生物医学工程生物医学工程
纳米技术纳米技术

背景情况:

植物生物成像面临着诸如叶绿素自发光和光散射等挑战,减少信号与背景比 (SBR).
光探针尺寸对植物运输和成像功效的影响还没有得到充分的研究.

研究的目的:

开发可调整尺寸的近红外II (NIR-II) 光探针,用于增强植物生物成像.
为了研究探针尺寸与植物组织透和成像性能之间的关系.

主要方法:

开发了由聚乙烯糖醇 (PEG) 工程设计的NIR-II供体-接受体-供体 (D-A-D) 探针 (CCNU1020) ,具有三种尺寸:SYH1 (170 nm),SYH2 (80 nm) 和SYH3 (60 nm).
评估探头进入速度,分布和信号与背景比 (SBR) 在Epipremnum Aureum叶子中.
评估了烟草马赛克病毒 (TMV) 感染的阿拉比多普西斯 (Arabidopsis thaliana) 的探针积累和监测.

主要成果:

与SYH1 (170 nm) 和SYH2 (80 nm) 相比,SYH3 (60 nm) 显示出最快的叶子进入和最平稳的静脉传播.
在叶状静脉成像中,SYH3获得了高SBR (~18.6),超过了经典的NIR-I内素绿 (ICG) (~3.0).
在TMV感染的Arabidopsis thaliana病变中有效积累SYH3,与GFP标记的结果相关.

结论:

优化探头大小对于在NIR-II地区高效的植物生物成像至关重要.
SYH3为高对比度植物血管成像和早期病毒性疾病诊断提供了一个有前途的工具.
这种PEG工程策略提升了植物生物成像能力.

关键词:

在NIR-II光成像技术中使用NIR-II光成像技术.在PEG工程方面,PEG工程植物生物成像植物生物成像植物病原体诊断诊断植物病原体诊断

更多相关视频

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Published on: February 24, 2014

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.