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Updated: Sep 19, 2025

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在人体血中定量Tuvusertib (M1774) 通过LC-MS/MS

Tien V Le^1,2, Robert A Parise¹, Julianne L Holleran¹

¹Cancer Therapeutics Program, UPMC Hillman Cancer Center, Pittsburgh, Pennsylvania, USA.

Biomedical chromatography : BMC

|June 9, 2025

概括

一个新的5分钟测量量量化了人体血中的ATR抑制剂tuvusertib,使用LC-MS/MS. 这种经过验证的方法通过测量用于癌症治疗的tuvusertib药理学来支持临床试验.

科学领域:

药理学药理学是指药理学的学科.
分析化学分析化学
癌症生物学癌症生物学

背景情况:

ATAXIA-telangiectasia和Rad3相关的 (ATR) 蛋白激酶对于DNA损伤反应至关重要.
Tuvusertib (M1774) 是一种ATR抑制剂,用于癌症治疗的临床开发.

研究的目的:

开发和验证一种快速和强大的生物分析试验,用于量化人体血中的tuvusertib.
为了支持临床研究,使得精确的tuvusertib的药理动力学分析.

主要方法:

液体染色学并联质谱法 (LC-MS/MS) 用于量化.
在Phenomenex Synergi Polar-RP柱上使用了5分钟的测试,涉及蛋白质沉和梯度化.
用SCIEX 6500+质谱仪进行检测,使用正态电喷射电离.

主要成果:

该试验显示出强大的性能,并符合FDA生物分析方法验证标准.
在5-5000 ng/mL的广泛动态范围内实现了量化.
为了提高准确性,使用了一个稳定的同位素内部标准.

结论:

已经建立了对人体血中的tuvusertib进行5分钟LC-MS/MS测定的验证方法.
这种试验适用于支持正在进行的和未来的tuvusertib临床试验中的药理动力学研究.

关键词:

在 LC-MS/MS 中使用.M177474 年的第一千七百七十四年人类血的人类血.药物动力学药物动力学管道的垂直.验证验证的时间

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