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对TA102和WP2的比较分析 uvrA(pKM101) 在增强的阿梅斯试验中检测尼托拉胺变异原体的菌株

Rushikesh M Shukla¹, Darshan T Valani¹, Chetan K Kajavadara¹

¹Department of Pharmacology and Toxicology, Zydus Research Centre, Zydus Lifesciences Limited, Ahmedabad, India.

Environmental and molecular mutagenesis

|June 9, 2025

概括

增强的阿梅斯试验 (EAT) 有效地识别了使用大肠杆菌WP2 uvrA ((pKM101) 的某些尼托胺的突变性. 然而,Salmonella typhimurium TA102对某些尼托胺具有有限的敏感性,这影响了其在综合性评估中的有用性.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
微生物学微生物学

背景情况:

艾姆斯试验对于评估化学变异性至关重要,特别是对于尼托胺等环境污染物.
增强的艾姆斯测试 (EAT) 通过使用多个菌株和代谢激活来提高灵敏度.
沙门氏菌型菌TA102已被认可用于检测氧化变异原体,但其在EAT中对尼托胺的表现尚未完全理解.

研究的目的:

在增强的阿梅斯试验 (EAT) 条件下,评估沙门氏菌Typhimurium TA102的突变性反应.
评估TA102对特定的尼特罗斯胺,N-尼特罗斯二甲基胺 (NDMA) 和1-cyclopentyl-4-nitrosopiperazine (CPNP) 的敏感性.

主要方法:

使用了增强的艾姆斯测试 (EAT) 协议.
测试了大肠杆菌WP2 uvrA(pKM101) 和沙门氏菌typhimurium TA102.2.的突变性反应.
使用NDMA和CPNP作为尼托拉胺阳性对照.
使用老鼠和子肝 S9 分数的内置代谢激活.

主要成果:

大肠杆菌WP2 uvrA(pKM101) 对NDMA和CPNP都表现出一致的突变性反应,与已建立的EAT数据保持一致.
沙门氏甲型菌TA102对NDMA显示出强烈的突变性反应.
TA102没有对CPNP表现出显著的突变性反应,这表明菌株特定的敏感性.

结论:

这些发现支持在EAT协议中继续使用大肠杆菌WP2 uvrA ((pKM101) 进行尼托胺致变性评估.
沙门氏甲型菌TA102由于其可变的灵敏度,对全面的尼托拉胺突变性测试具有有限的效用.
可能需要进一步的研究来优化TA102的应用或确定用于特定变异原体检测的替代菌株.

关键词:

1 - cyclopentyl-4-nitrosopiperazine 的使用情况.大肠杆菌WP2 uvrA ((pKM101)) 的情况.这是一种N-Nitrosodimethylamine.S. typhimurium TA1022 是一种类型的植物.增强的艾姆斯试验条件汉姆斯特肝 S9 汉姆斯特肝 S9 汉姆斯特肝鼠肝S9 鼠肝S9 是一个

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