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Updated: Sep 19, 2025

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
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Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

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一个基于单链抗体的AID2系统,用于条件降解GFP标记和未标记蛋白质

Moutushi Islam1,2, Takefumi Negishi2,3, Naomi Kitamoto1,4

  • 1Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS), Yata 1111, Mishima, Shizuoka 411-8540, Japan.

Journal of cell science
|June 9, 2025
PubMed
概括

我们开发了一种新的单链抗体AID2 (scAb-AID2) 系统,用于蛋白质敲击. 这项技术扩大了辅酶诱导降解 (AID2) 技术的应用范围,用于研究各种生物体中的蛋白质功能,并推进治疗方法.

关键词:
AID2 AID2 的意思是帮助.一个体的单体.一个纳米人体.单链抗体是一种单链抗体.有针对性的蛋白质降解降解.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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