对Ti,Zr和Hf氧化物的替代消化策略:消除酸
Lukas R H Gerken1,2, Matthias Roesslein1, Inge K Herrmann1,2,3,4
1Laboratory for Nanomaterials in Health, Department of Materials Meet Life, Swiss Federal Laboratories for Materials Science and Technology (Empa), Lerchenfeldstrasse 5, St. Gallen, 9014, Switzerland. alexander.gogos@empa.ch.
概括
一种新的无酸微波消化方法安全量化生物样本中的IV组金属氧化物. 这种方法实现了高回收率,为毒性和生物分布研究提供了更安全的替代方案.
科学领域:
- 环境科学 环境科学
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
背景情况:
- 第四组金属氧化物 (例如,TiO2,ZrO2,HfO2) 广泛使用,但由于它们的惰性,在生物矩阵中难以量化.
- 准确的量化对于评估环境影响,人类健康风险,生物分布和毒性至关重要.
- 传统方法经常使用危险的酸 (HF),需要专门的安全协议.
研究的目的:
- 开发和验证一个安全的,没有酸 (HF) 的微波辅助消化协议,用于生物样本中的IV组金属氧化物.
- 为了能够准确量化纳米到微尺度的IV组金属氧化物,用于环境和生物医学应用.
- 为现有的基于HF的消化技术提供更容易获得和更安全的替代方案.
主要方法:
- 开发了一种使用硫酸,水和过氧化混合物的无HF微波消化协议.
- 在各种商用TiO2,ZrO2和HfO2粉末 (纳米到微观) 上测试了该协议.
- 优化了消化参数 (酸比,温度,时间),并使用感应合等离子体光学发射谱法 (ICP-OES) 进行验证.
主要成果:
- 对所有经过测试的IV组金属氧化物实现了>90%的回收,对于更大的颗粒和更高原子数的氧化物需要更高的温度/时间.
- 在人类状癌细胞和牛肝组织中成功量化,检测极限约为1ppb和80-100%的恢复.
- 样品的稳定性在消化后长达四周保持.
- 无HF方法显示了与基于HF的方法相比较的灵敏度和检测极限.
结论:
- 开发的无HF微波消化协议对复杂的生物矩阵中IV组金属氧化物的完全溶解和精确量化是有效的.
- 该方法为例行实验室分析提供了更安全,更容易获得和验证的替代方案,支持环境和生物医学研究.
- 促进对IV组金属氧化物的生物分布和毒性进行关键研究,而无需使用HF相关的风险.
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