孕产妇的vGluT2和胚胎的mGluR3信号继电系统控制了 pea aphid 中的后代翅膀模态
Yiyang Yuan1,2, Yanyan Wang2,3, Wanwan Ye2,3
1Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, Shandong Province 250100, China.
iScience
|June 9, 2025
概括
母亲拥挤信号触发了虫的翅膀发育. 这通过大脑囊泡性谷氨酸转运体2和胚胎性转基因谷氨酸受体3发生,从而影响后代的翅膀模态.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 虫翅膀二态化是跨代表型可塑性的例子.
- 控制这种可塑性的母体信号通路在很大程度上是未知的.
研究的目的:
- 阐明母体身体接触影响豆虫翅膀发育的分子机制.
主要方法:
- RNA测序 (RNA-seq) 用于识别差异表达的基因.
- 基因淘汰实验用于评估功能角色.
- 电泳运动转移试验 (EMSA) 测定蛋白质-DNA相互作用.
主要成果:
- 身体接触和拥挤增加了大脑膀性谷氨酸转运体2 (ApvGluT2) 和胚胎代谢基因谷氨酸受体3 (ApmGluR3) 的表达.
- 在胚胎中,阻断ApvGluT2或ApmGluR3破坏了ApFoxO酸化.
- 脱化ApFoxO直接抑制了刺 (ApHh) 转录,减少了有翅膀的后代.
结论:
- ApvGluT2和ApmGluR3作为母体拥挤信号的关键媒介.
- 这些基因协调后代的翅膀发育,揭示了跨代可塑性的新机制.
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