相关实验视频

Updated: Aug 6, 2026

Identification of Protein Interacting Partners Using Tandem Affinity Purification
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Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

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一种精简的 His-MBP-SpyCas9 协同亲和度净化,没有缓冲区交换,适用于体外裂解应用

Filippo Fronza1, Roberto Verardo2,3, Claudio Schneider1,2

  • 1DME Dipartimento di Medicina, University of Udine, Piazzale Kolbe 1, 33100 Udine, Italy.

MethodsX
|June 9, 2025
PubMed
概括

我们开发了一个快速的,一天的协议,用双亲关系染色学净化His-MBP-SpyCas9蛋白质. 这种方法简化了净化,并产生了准备用于体外测试的高质量Cas9蛋白.

关键词:
阿克塔开始开始克里斯普尔是什么意思?克里斯普尔是什么意思?这就是Cas9的情况.Cas9 双联亲和性净化方法这就是FPLC.他的标签是他的标签.他的TRAP 陷这是一个MBP标签.MBPTrap 的意思是 MBPTrap.蛋白质净化 蛋白质的净化这就是SpCas9的意义.这就是SpyCas9的原因.

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