修改QuEChERS用于法医毒理学中的抗药物检测
Ahmad Shekari1, Jalal Hassan1, Mohammad Kazem Koohi1
1Division of Toxicology, Department of Comparative Bioscience, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
这项研究开发了一种快速,廉价和绿色的QuEChERS方法,用于分析死后血清中的抗药物. 经过验证的技术为法医毒理学应用提供了准确的药物度测量.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学 是一个学科.
背景情况:
- 在尸体样本中精确量化抗药物 (AED) 对于法医调查至关重要.
- 传统的AED分析方法可能耗时,昂贵,并且可能需要大量的样本.
- 开发高效可靠的分析技术对于死后药物查至关重要.
研究的目的:
- 开发和验证一种改进的QuEChERS (快速,轻松,廉价,坚固和安全) 提取方法,用于在死后血清中同时测定巴比他,卡巴马西平,普里米和伊.
- 评估法医毒理学分析的拟议方法的效率,准确性和检测极限.
- 为AED量化建立一种廉价,快速和环保的方法.
主要方法:
- 用修改的QuEChERS程序提取血清样本.
- 提取物的净化使用硫酸,PSA (初级二级氨基) 和C18吸附剂进行.
- 分析使用高性能液态染色学与紫外线检测 (HPLC/UV) 进行.
- 研究了样品pH值对提取效率的影响.
主要成果:
- 目标AED的提取回收率从42%到97%不等.
- 奎瑟斯 (QuEChERS) 方法证明了高提取效率,产生了更清洁,更纯净的样品.
- 该方法实现了良好的分析效率,平均准确度为70-85%,回归系数> 0.99.
- 发现检测极限在0.21-0.38 ng/mL之间.
结论:
- 修改后的QuEChERS方法是一种合适,具有成本效益和快速的技术,用于在死后血清中确定AED.
- 开发的方法提供了高样本吞吐量,被认为是绿色化学方法.
- 这种经过验证的方法是法医毒理学中药物分析的首选选择,因为它的效率和纯度.
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