相关实验视频

Updated: Sep 19, 2025

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

Published on: August 14, 2018

一种具有膜融合介导病毒杀伤能力的广谱抗病毒阶段可转化脂质体

Yutong Li^1,2, Yuhang Li¹, Xu Li²

¹Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, P. R. China.

Advanced healthcare materials

|June 9, 2025

概括

一种新型的硫化脂质体 (SC_Lip) 通过pH诱导的膜融合有效地使病毒失活,提供广泛的抗病毒保护. 这种方法可以在各个阶段对抗病毒感染,降低再感染风险,为流行病准备工作铺平道路.

科学领域:

生物材料科学生物材料科学
病毒学病毒学
纳米技术纳米技术

背景情况:

病毒感染对全球健康构成重大威胁,需要广泛的抗病毒解决方案.
目前的抗病毒药物针对特定的病毒,突出显示了对多功能治疗策略的需求.
肝素硫酸蛋白质甘 (HSPG) 仿生策略对广泛的抗病毒药物有希望,但可逆结合带来了再感染的风险.

研究的目的:

开发一种具有增强疗效和降低再感染风险的新型广谱抗病毒材料.
为了研究pH触发的脂肪体相变的潜力,用于病毒失活.
评估开发的硫化脂质体 (SC_Lip) 的广泛抗病毒活性和体内疗效.

主要方法:

选和设计一种硫化脂质体 (SC_Lip),能够在pH值6.5以下的pH值诱导相位过渡.
通过SC_Lip的相变触发的膜融合来研究病毒无活化的机制.
测试SC_Lip对各种病毒 (SARS-CoV-2,LV,HSV-1) 的广泛杀菌活性.
评估体内抗病毒功效,使用老鼠HSV-1前膜炎模型.

主要成果:

SC_Lip证明了pH诱导的相位过渡,导致膜与病毒包裹的融合以及随后的无活化.
证实了针对冠状病毒 (SARS-CoV-2),逆转录病毒 (LV) 和疹病毒 (HSV-1) 的广泛杀菌活性.
在体内研究验证了SC_Lip在HSV-1前膜炎的老鼠模型中的抗病毒作用.

结论:

通过诱导膜融合和病毒失活,SC_Lip提供了一个有前途的广泛抗病毒策略.
这种pH触发机制有效地在酸性环境中对抗病毒感染,包括细胞内溶解体.
由于其广泛的疗效和降低了再感染风险,SC_Lip为打击新出现的病毒大流行提供了潜在的解决方案.

关键词:

在HSPG生物仿真策略中.广泛的抗病毒药物.膜融合融合是什么膜融合是什么阶段转化性脂质细胞体病毒失活是病毒的失活.

更多相关视频

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

相关概念视频

Retroviruses

Retroviruses

Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...

Viral Structure

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.

Retrovirus Life Cycles

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...

Subviral Agents

Subviral Agents

Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...