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活跃的子形成的有约束性厌氧生物在巴氏消毒的捐赠人乳中很罕见:试点研究
Tong Wu1, Laura D Klein1, Scott Markham1
1Australian Red Cross Lifeblood, West Melbourne, Australia.
概括
化供体人乳 (PDHM) 对早产婴儿是安全的,因为在化后很少发现无氧细菌. 对于PDHM进行常规无氧检测并不是必不可少的,特别是冷储存.
科学领域:
- 微生物学 微生物学
- 新生儿营养新生儿营养
- 食品安全 食品安全
背景情况:
- 纯化捐赠人母乳 (PDHM) 是高风险早产婴儿的重要营养来源,当母亲自己的牛奶无法获得时.
- 对PDHM的微生物查对于安全至关重要,因为一些细菌,如义务性无气体 (例如,Clostridium物种),可能会在巴氏杀菌过程中存活下来.
- 目前的全球牛奶储存指南建议进行微生物查,但缺乏特定的无氧检测协议.
研究的目的:
- 为了评估可活性厌氧细菌在巴氏消毒后捐赠人乳 (PDHM) 批次中的流行率.
- 评估PDHM安全协议中常规无氧测试的必要性.
主要方法:
- 一项前性队列研究,涉及来自独特捐赠者的150批PDHM.
- 样品使用经过验证的方法测试有氧和无氧细菌,检测极限为≤1CFU/mL.
- 化前的样本进行了有氧检测; 化后的样本进行了有氧和无氧检测.
主要成果:
- 在150个巴氏消化后PDHM样本中,没有检测到任何可活的无氧细菌.
- 消毒前查发现4.7%的样本未能满足有氧细菌计数指南.
- 没有任何巴氏消化后样本通过了有氧或无氧测试.
结论:
- 强制性厌氧细菌很少从PDHM培养,这表明它们在巴氏消毒后不会构成重大风险.
- 常规无氧检测PDHM不被认为是必不可少的,特别是在冷 (<18°C) 储存时.
- 医疗保健提供者应该保持警,寻找接受者的不良事件,并向牛奶银行报告它们.
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