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与脂肪肝病患者相比,健康个体的标准饮食对肝转录组的调节不同
Josephine Grandt1,2, Christian D Johansen2, Anne-Sofie H Jensen1,2
1Gastro Unit, Copenhagen University Hospital Hvidovre, Denmark.
PloS one
|June 9, 2025
概括
肝脏 肝脏是肝脏中的一个部分.
科学领域:
- 肝病学和转录学.
- 分子生物学和生物信息学
背景情况:
- 人的肝脏适应食,但食物摄入如何影响非酒精性脂肪肝 (NAFLD) 和肝硬化中的肝脏基因表达是未知的.
- 之前的研究表明,与对照人群相比,NAFLD和肝硬化患者的转录组特征发生了变化.
研究的目的:
- 在NAFLD,肝硬化和健康个体中调查快食和食后状态的肝转录组差异.
- 创建一个可浏览的在线资源,用于肝脏转录组数据.
- 为了测试食物摄入不同调节这些群体中的肝转录组的假设.
主要方法:
- 通过从NAFLD (n=9),肝硬化 (n=9) 和健康对照 (n=10) 获得的横活检获得的肝脏组织.
- 下一代测序 (NGS) 用于肝转录组分析.
- 生物信息分析用于识别差异表达基因 (DEG) 和基因本体生物学过程 (GOBP).
主要成果:
- 在所有组中发现了显著的DEG:对照组和NAFLD之间553例,对照组和肝硬化之间5527例,NAFLD和肝硬化之间3898例.
- 在NAFLD和肝硬化中,MET原瘤基因的表达有差异.
- 标准化食诱导了对照组的肝转录组显著变化,但这些变化在NAFLD和肝硬化患者中变得淡.
- 在NAFLD和肝硬化中,增多了亲炎性/亲纤维性基因和降低了与代谢相关的基因.
- 数据可以通过两个基于Web的应用程序访问.
结论:
- 与NAFLD和肝硬化患者相比,健康个体的食物摄入不同调节肝转录组.
- NAFLD和肝硬化与基因表达的改变有关,包括炎症/纤维化增加和新陈代谢减少,在饭后进一步失调.
- 开发的资源为进一步研究肝病提供了可访问的数据.
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