生物能量压力增强了抗微生物耐药性和持久性
Barry Li1,2, Shivani Srivastava1,2, Mustafa Shaikh1,2
1Center for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical School, Newark, NJ, USA.
Nature communications
|June 9, 2025
概括
大肠杆菌中的生物能量压力通过增加活性氧物种 (ROS) 和激活修复机制来增强抗生素耐药性和持久性. 这项研究揭示了细胞能量平衡如何影响细菌抗生素生存.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 抗生素的作用可能涉及增加细胞能量需求,包括ATP消耗,呼吸和反应性氧物种 (ROS) 生成.
- 了解影响抗生素耐药性和持久性的因素对于对抗细菌感染至关重要.
研究的目的:
- 研究生物能压力对大肠杆菌抗生素疗效的影响.
- 阐明生物能压力影响抗生素耐药性和持久性的机制.
主要方法:
- 通过大肠杆菌中ATP和NADH的构成性水解诱导生物能压力.
- 分析机制,包括ROS生产,突变性断裂修复和转录合修复.
- 与抗生素持久性相关的严格反应的评估.
主要成果:
- 生物能压力增强了抗生素耐药性的演变.
- 增强的ROS产生,突变性断裂修复和转录合修复有助于在压力下抵抗.
- 生物能压力通过激活严格反应增加了抗生素持久性.
结论:
- 细胞生物能状态显著调节细菌对抗生素的反应.
- 提出了一个模型,其中ATP消耗/生产平衡调节了抗生素耐药性和持久性.
- 准细胞能量通路可能是克服抗生素耐药性的策略.
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