来自深海衍生真菌的新型抗微生物类物质Aspergillus tabacinus LW82 LW82
Yu Tu1, Jinxin Zhang1,2, Lei Cai1,2
1State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
The Journal of antibiotics
|June 9, 2025
概括
深海真菌Aspergillus tabacinus产生了新的化合物阿斯伯丁化物A和B,以及合物C.化合物6对Cryptococcus gattii表现出强大的抗真菌活性.
科学领域:
- 自然产品化学 自然产品化学
- 海洋微生物学 海洋微生物学
- 菌类代谢学 菌类代谢学
背景情况:
- 深海环境是新型微生物的丰富来源,具有独特的代谢能力.
- 亚斯伯吉洛菌种是众所周知的多种生物活性二次代谢产物的生产者.
- 一个菌株多种化合物 (OSMAC) 策略增强了微生物天然产品的发现.
研究的目的:
- 从深海衍生的真菌中分离和鉴定新的生物活性化合物 Aspergillus tabacinus LW82.
- 评估分离化合物的抗微生物和抗真菌活性.
主要方法:
- 使用乙酸乙烯原料提取物以OSMAC策略为指导的化合物的分离.
- 使用综合光谱分析 (NMR,HRESIMS,ECD) 进行结构阐明和绝对配置确定.
- 确定抗菌和抗真菌检测的最小抑制度 (MIC) 值.
主要成果:
- 发现了两种新的布他诺化物,即阿斯珀布他诺化物A (1) 和B (2),以及一种新的脂肪酸衍生物,即法洛托尔C (3).
- 化合物1证明了对Pseudomonas syringae pv.的抗菌活性. 拉克里曼 (MIC: 64微克/毫升).
- 化合物6对Cryptococcus gattii (MIC: 8μg/mL) 具有显著的抗真菌功效,与可纳相当.
结论:
- 深海真菌Aspergillus tabacinus LW82产生新的生物活性布他诺化物和脂肪酸衍生物.
- 这些已识别的化合物具有有前途的抗菌和抗真菌特性,突出显示了它们作为治疗药物的潜力.
- 在发现海洋衍生真菌的新代谢物方面,OSMAC战略被证明是有效的.
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