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单细胞染色体可访问性分析揭示了猪大脑中的调节机制和演变

Yue Xiang¹, Saixian Zhang¹, Yi Huang¹

¹Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, P.R. China.

|June 9, 2025

概括

猪的大脑提供了对人类神经系统状况的宝贵见解. 这项研究揭示了保存的调节元素和疾病变异丰富,表明猪可能比小鼠更好地模拟阿尔茨海默病.

科学领域:

神经科学是一个神经科学.
基因组学就是基因组学.
比较生物学的比较生物学

背景情况:

猪的大脑在结构上与人类大脑相似,使它们成为有价值的生物医学研究模型.
化已经显著改变了猪的大脑,提供了独特的研究机会.
由于细胞异质性,了解猪大脑调节机制至关重要.

研究的目的:

为了研究猪大脑中细胞类型特定的调控场景.
为了比较家养猪和野猪之间的监管要素和进化速率.
评估猪作为人类大脑疾病模型的实用性.

主要方法:

来自猪和野猪大脑皮质和小脑的71,798个细胞的单细胞RNA测序.
整合数据以探索监管格局和寡头细胞的发展.
用人类和小鼠基因组进行跨物种比较分析.

主要成果:

确定了九种不同的细胞类型,并探索了细胞类型特定的调控元素.
在家养猪中,寡头质细胞前代细胞显示出潜在的更快的进化速度.
猪表现出比小鼠更高比例的保存调节元件,对免疫和神经疾病,包括阿尔茨海默病,具有丰富的变体.

结论:

提供了关于猪大脑异质性和调节机制的初步见解.
强调神经系统差异对表型变化的潜在影响.
建议猪可能比小鼠更适合用于阿尔茨海默病研究的模型.

关键词:

生物医学模型的模型.脑子脑子脑子脑子关联监管要素关联监管要素这些猪是猪.这就是 ScATAC-seqq.

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