相关实验视频
Updated: Jun 13, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
TGF-β通过调节转录因子,诱导甲状腺癌细胞中的EMT
Jianjian Xiang1, Nannan Lv2, Shanyu Yin1
1Department of Ultrasound Medicine, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.
转化生长因子-β (TGF-β) 在甲状腺癌 (TC) 细胞中促进上皮-介质细胞转换 (EMT),增加侵袭和迁移. 甲状腺刺激激素 (TSH) 增强了这种TGF-β诱导的EMT过程.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 内分泌学 在内分泌学.
背景情况:
- 转化生长因子-β (TGF-β) 与癌症侵袭和上皮-介质细胞转变 (EMT) 有关.
- 在甲状腺癌 (TC) 中TGF-β的特定作用及其潜在机制需要进一步阐明.
研究的目的:
- 研究甲状腺癌细胞中TGF-β对EMT的影响.
- 确定TGF-β影响TC中的EMT的分子机制.
主要方法:
- 甲状腺癌细胞系被TGF-β治疗.
- 免疫光染色评估了E-cadherin的局部化.
- 伤口愈合和Transwell测试评估了细胞迁移和入侵.
- 分析了EMT标记物 (E-cadherin,N-cadherin,vimentin,SNAI1) 的mRNA和蛋白质水平.
- 还检查了TSH的影响.
主要成果:
- 治疗TGF-β诱导了形态变化,并在细胞膜下降了E-cadherin.
- 在TGF-β处理的细胞中,细胞迁移和侵入显著增加.
- TGF-β上调SNAI1,N-cadherin和维丁,同时在mRNA和蛋白质水平上降低E-cadherin的调节.
- 发现甲状腺刺激激素 (TSH) 可以增强这些TGF-β诱导的EMT过程.
结论:
- TGF-β有效地促进甲状腺癌细胞中类似EMT的表型变化.
- 该研究确定了SNAI1和关键EMT标记物的上调作为涉及的机制.
- 在TC中,TSH增强了TGF-β驱动的EMT,这表明TC治疗的潜在治疗点.
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