线素激活蛋白激酶7酸化转录因子ZmWRKY104以提高玉米的盐分耐受性
Jing Li1, Chen Zhang1, Yadan Miao1
1College of Life Sciences, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Journal of experimental botany
|June 10, 2025
概括
一种新发现的蛋白质激酶,ZmMAPK7,通过激活ZmWRKY104.4来提高玉米盐的耐受性. 这种相互作用增强了超氧化物脱酶活性,减少了有害的活性氧物种,并改善了植物在盐应激下的生存.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 盐应激严重限制了全球的作物生产率.
- WRKY转录因子ZmWRKY104通过激活ZmSOD4.4来增强玉米盐的耐受性.
- 在盐反应中控制ZmWRKY104的上游监管机制仍然不清楚.
研究的目的:
- 在玉米盐耐受性中识别和描述ZmWRKY104-ZmSOD4模块的上游调节器.
- 阐明ZmMAPK7影响玉米盐反应的分子机制.
主要方法:
- 酵母双杂交和共免疫沉试验以确认蛋白质相互作用.
- 酶活性测定用于测量超氧化脱酶 (SOD) 活性.
- 酸化位点分析和促进体结合试验.
主要成果:
- ZmMAPK7被确定为一个上游调节器,它与ZmWRKY104.4物理相互作用.
- ZmMAPK7通过增加SOD活性和减少超氧化离子积累来提高玉米盐耐受性.
- 在Ser142中酸化ZmMAPK7ZmWRKY104,增强其促进体结合和ZmSOD4激活.
结论:
- ZmMAPK7在ZmWRKY104的上游作用,通过一种新的调节途径来增强玉米的盐耐受性.
- 这一途径涉及ZmMAPK7-介导ZmWRKY104的酸化,从而改善ZmSOD4的表达和功能.
- 了解这种机制为提高作物对盐度压力的抵抗力提供了新的目标.
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