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Updated: May 6, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

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了解基于粒子的竞争性连续生物传感器中的快速和慢速信号变化

Sebastian Cajigas1, Arthur M de Jong2,3, Junhong Yan4

  • 1Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ, Eindhoven, The Netherlands.

Analytical chemistry
|June 10, 2025
PubMed
概括

这项研究调查了糖类生物传感器中依赖时间的信号变化. 快速变化与粒子-表面相互作用有关,而缓慢的变化源于分子解离,告知长期连续传感.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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