相关实验视频

Updated: Sep 19, 2025

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Published on: February 17, 2022

通过光学和PSMA准的PET成像开发一个现场CAR-T细胞协议

Nisi Zhang¹, Jai Woong Seo¹, Elise Robinson¹

¹Molecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Stanford, CA 94304.

Proceedings of the National Academy of Sciences of the United States of America

|June 10, 2025

概括

这项研究介绍了一种新的在位化学抗原受体 (CAR) -T细胞疗法,使用脂质纳米颗粒在体内设计T细胞. 这种方法可以通过正子发射断层扫描 (PET) 图像进行有效的瘤消除和跟踪.

科学领域:

免疫治疗是一种免疫疗法.
纳米技术纳米技术
医疗成像医学成像

背景情况:

传统的仿真抗原受体 (CAR) -T疗法复杂且昂贵.
在现场CAR-T细胞生成需要先进的成像用于监测,因为缺乏ex vivo质量控制.

研究的目的:

开发和验证用于癌症治疗的现场T细胞工程方法.
通过使用正子发射断层扫描 (PET) 实现在位生成的CAR-T细胞的跟踪和功能评估.

主要方法:

使用抗CD5结合脂质纳米颗粒来输送CD19 CAR mRNA (mCAR19) 和前列腺特异性膜抗原mRNA (mPSMA) 标签用于in situ T细胞工程.
纳米颗粒的使用interleukin-7 (IL-7) 预先调节和重复管理.
使用PET成像与Ga-PSMA-617追踪工程T细胞.

主要成果:

在75%的B细胞淋巴瘤携带小鼠中实现了无瘤生存,与实体CAR-T疗法相比.
通过PET成像验证了现场工程,PSMA标记的CD19 CAR-T细胞的生成和瘤透.

结论:

在现场开发的CAR-T细胞生成和跟踪方法为传统的ex vivo方法提供了一个有前途的替代方案.
这种技术允许在体内有效监测CAR-T细胞疗法,解决当前方法中的一个关键挑战.

关键词:

在CAR-T疗法中,在这里,PET是PET.免疫疗法免疫疗法脂质纳米颗粒的使用方法在mRNA疗法中使用的mRNA疗法

更多相关视频

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

相关概念视频

Positron Emission Tomography

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...