在血液采样管中分离剂凝对 perfluoroalkyl 和 polyfluoroalkyl 物质分析的影响
Patrice Dufour1,2, Catherine Pirard1,2, Corinne Charlier1,2
1Department of Clinical, Forensic and Environmental Toxicology, University Hospital of Liege (CHU Liège), CHU (B35), 4000 Liege, Belgium.
Laboratory medicine
|June 10, 2025
概括
推使用凝块激活器管 (CAT) 进行精确的和多醇基物质 (PFAS) 测试. 含有凝的管道可能会因为吸附而低估PFAS水平,影响临床实验室结果.
科学领域:
- 环境化学环境化学
- 临床诊断 临床诊断 临床诊断
- 分析化学 分析化学
背景情况:
- 越来越多的人担心对和多基基物质 (PFAS) 的担忧,需要可靠的分析方法.
- 目前对PFAS量化的建议建议不要使用血清分离管,因为它可能吸附于凝.
- 凝吸附对PFAS准确性的影响尚未评估.
研究的目的:
- 为了评估血清分离器管凝对和多醇基物质 (PFAS) 测量的准确性的影响.
- 为了比较无凝凝块激活管 (CAT) 与含有凝的血清分离管中的PFAS度.
主要方法:
- 添加PFAS的血液样本被储存在CAT和血清分离管中长达24小时.
- 分析PFAS度 (16种化合物) 使用液体染色学-并联质谱法.
- 计算了管类型之间的PFAS水平的百分比变化.
主要成果:
- 大多数PFAS在24小时内在两种管类型中都显示出极小的变化 (<5%).
- 长链氧硫酸盐,包括氧硫酸盐 (PFOS),表现出凝吸附.
- 在血清分离管中,与CAT相比,观察到PFOS的统计学上显著低估 (P < .001).
结论:
- 建议使用凝血激活管 (CAT) 进行PFAS分析,以防止低估.
- 血清分离管可能导致由于凝吸附导致不准确的PFAS量化.
- 在临床实验室进行准确的PFAS测试对于监测和风险评估至关重要.
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