聚乙烯纳米塑料通过通过细胞外膀传递的微RNAs改变细菌与宿主相互作用来破坏肠道微环境
Wei-Hsuan Hsu1,2, You-Zuo Chen1, Yi-Ting Chiang1
1Department of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Nature communications
|June 10, 2025
概括
纳米塑料 (NP) 通过改变微RNA和破坏肠道细胞来破坏肠道屏障. 这导致透性增加和肠道功能失调,对人类健康构成风险.
科学领域:
- 环境健康 环境健康
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 纳米塑料 (NP) 是新兴的环境污染物.
- 对NP对人类健康的潜在风险尚未完全理解.
- 肠道屏障功能对健康至关重要,并可能受到环境因素的影响.
研究的目的:
- 调查聚烯-纳米塑料暴露如何破坏肠道微环境.
- 阐明细菌与宿主相互作用在纳米塑料引起的肠损伤中的作用.
- 确定特定的分子机制,包括微RNA变化和微生物群变化.
主要方法:
- 在体内小鼠模型被用于研究纳米塑料暴露.
- 使用细菌分类技术分析了细菌与宿主之间的相互作用.
- 测量了特定的微RNA (miRs) 和蛋白质 (ZO-1,MUC-13) 的表达水平.
- 分析了来自杯细胞的细胞外囊泡 (EVs).
- 评估了肠道微生物群的组成.
主要成果:
- 纳米塑料积累改变了肠道miR-501-3p和miR-700-5p的表达,损害了ZO-1和MUC-13,增加了肠道的透性.
- 纳米塑料增加了杯状细胞衍生的EV中的特定miRs,干扰ZO-1.
- 诱导了肠道微生物群失调,并增加了Ruminococcaceae.
- 拉克诺斯皮拉ceae内化纳米塑料,他们的EV抑制了MUC-13表达.
结论:
- 纳米塑料通过改变的microRNA表达和受损的屏障蛋白质损害了肠道完整性.
- 纳米塑料诱导肠道失调并改变细胞外囊泡的功能.
- 细菌与宿主相互作用中的这些干扰揭示了纳米塑料诱导的肠损伤和潜在的不良健康结果的机制.
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