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Ghr-miR166b-GhHB14 配对与 GhDLO1 合作伙伴调解棉花植物对抗垂直达利亚的防御

Yuan Meng^1,2, Ge Zhao¹, Ye Tang³

¹School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.

Molecular plant pathology

|June 10, 2025

概括

一种特定的微RNA (miRNA) 作为棉花植物对抗Verticillium wilt.的防御增强剂. 这种miRNA向一种基因 (GhHB14),该基因与另一种蛋白质 (GhDLO1) 一起,通常会抑制植物对这种疾病的抵抗力.

科学领域:

植物分子生物学植物分子生物学
植物病理学植物病理学
遗传学遗传学是一个

背景情况:

第三类家庭主体-素拉链 (HD-ZIP III) 转录因子对植物发育至关重要.
Verticillium枯对全球棉花生产构成重大威胁,但抵抗的潜在分子机制尚未完全理解.
关于HD-ZIP III参与植物病原体相互作用的研究,特别是在棉花和Verticillium枯中,仍然有限.

研究的目的:

调查棉花HD-ZIP III转录因子GHB14及其由ghr-miR166b调节的作用,以保护棉花免受Verticillium wilt.
阐明棉花对Verticillium dahliae.的反应所涉及的分子相互作用和调节途径.

主要方法:

降级组测序以确定miRNA-目标相互作用.
快速放大cDNA末端 (RACE) 测序以确认目标部位.
在体内光测试以验证miRNA向.
酵母两杂交和双分子光补充 (BiFC) 试验用于研究蛋白质-蛋白质相互作用.
基因沉默技术 (例如RNA干扰) 来评估植物中关键基因的功能.
病原体感染测定用于评估植物耐药性.

主要成果:

ghr-miR166b在特定位置直接准并分裂GHB14mRNA,这表明了转录后调节.
GhHB14在植物细胞内与GHDLO1进行物理相互作用.
抑制ghr-miR166b导致对Verticillium dahliae的敏感性增加.
相反,对 GhHB14 或 GhDLO1 的沉默导致对 Verticillium dahliae 感染的抗性显著增强.
ghr-miR166b充当正调节剂,而GhHB14和Ghdlo1则充当棉花防治Verticillium wilt.的负调节剂.

结论:

ghr-miR166b-GhHB14调节轴与Ghdlo1一起,在调节棉花对Verticillium dahliae的防御反应方面发挥着至关重要的作用.
ghr-miR166b通过降低负调节剂 GhHB14 和 GhDLO1.1 的下调来增强棉花的抗性.
这项研究揭示了一种新的miRNA介导的调节机制,可以控制棉花中的植物免疫力.

关键词:

这种植物名为Verticillium dahliae.在 GhDLO1 的位置上.在GHB14,GHB14中使用.棉花棉花是一种棉花.ghr-miR166bb 在线阅读

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