羔羊卵巢和卵细胞的转录体表征揭示了超排卵后的关键生物标志物
Jingjing Wang1, Jiachen Bai2, Yucheng Liu1
1State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Institute of Animal Husbandry and Veterinary Sciences, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
Journal of animal science
|June 11, 2025
概括
羊羔的青少年体外胚胎移植 (JIVET) 显示了较低的卵细胞发育率,而不是成年人. 在PI3K/Akt通路中的关键蛋白质ERBB3和HRAS升高,表明卵细胞能力降低.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 分子生物学分子生物学
背景情况:
- 过度排卵的青春期前羔羊用于青少年体外胚胎移植 (JIVET).
- 绵羊卵巢对淋巴激素的反应不同于成年雌羊,影响卵细胞质量.
- 卵巢和卵细胞发育状态对于卵泡招募和成熟效率至关重要.
研究的目的:
- 为了调查羊羔和成年绵羊卵细胞在体外发育速率中的差异.
- 在超卵性羔羊中,确定导致卵细胞发育能力下降的分子机制.
主要方法:
- 在体外对卵子细胞的发育评估 (成熟,分裂,胚胎细胞形成).
- 卵巢的全转录组测序 (RNA-seq) 和卵细胞的超低RNA-seq.
- 综合性转录基因分析以识别差异表达基因 (DEG).
- 免疫光和免疫组织化学分析蛋白质表达 (ERBB3,HRAS).
主要成果:
- 与成年绵羊卵细胞相比,绵羊卵细胞的成熟,裂变和芽细胞形成率明显较低 (P < 0.05).
- 综合性转录基因分析揭示了DEGs中的PI3K/Akt信号通路的丰富.
- 在超卵性羔羊的卵巢卵泡和卵细胞中观察到ERBB3和HRAS蛋白的表达升高.
结论:
- ERBB3和HRAS是降低超卵性羔羊卵细胞发育能力的关键生物标志物.
- PI3K/Akt信号通路在观察到的卵细胞质量差异中起着重要作用.
- 了解这些分子差异可以提高羔羊的JIVET效率.
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