使用新HPLC方法对金科比洛巴提取物进行定量分析:用于确定多个成分的单一标准
Jianing Li1, Ping Li1,2, Hao Sun1,3
1SKL of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian, Liaoning 116034, China.
Journal of chromatographic science
|June 11, 2025
概括
开发了一种使用高性能液态染色学对金科比洛巴叶 (GBL) 进行质量控制的新方法. 这种方法准确量化了12种主要的黄,为现有技术提供了可靠的替代方案.
科学领域:
- 植物化学 植物化学
- 分析化学 分析化学
- 药理学是指药理学,即药理学是指药理学.
背景情况:
- 银杏叶 (GBL) 由于其药用特性而广泛使用,主要归因于其含有黄类的成分.
- 精确的GBL质量控制对于确保产品的有效性和安全性至关重要.
- 在GBL中量化黄类的现有方法可能是耗时和复杂的.
研究的目的:
- 开发和验证一种新的,高效的质量控制方法,用于同时确定GBL中的12种主要黄类素.
- 将新方法的性能与传统的外部标准 (ES) 和中国药典 (CP) 方法进行比较.
- 为GBL分析建立一个单一的多元组件 (SSDMC) 确定标准.
主要方法:
- 使用高性能液态染色学 (HPLC) 进行了分析.
- 开发了一种使用单一标准来确定多个组件 (SSDMC) 的新型质量控制方法.
- 验证包括线性,检测和量化极限,精度,准确性,可重复性和稳定性评估.
主要成果:
- 开发的SSDMC方法显示出优秀的验证参数:线性 (r2 > 0.9993),低检测极限 (0.13-0.83μg/mL) 和量化 (0.43-2.76μg/mL).
- 实现了高精度 (RSD < 0.89%),准确性 (92-114%),可重复性 (RSD < 1.92%),以及稳定性 (RSD < 1.96%).
- 通过SSDMC和ES方法确定的黄酸含量没有显著差异,而CP方法的结果略低.
结论:
- 新的基于HPLC的SSDMC方法是一种可信和可行的方法,可以同时确定GBL中的12种主要黄类素.
- 这种方法为GBL质量控制提供了一个高效可靠的替代方案.
- 这些发现支持使用SSDMC进行标准化GBL提取物分析.
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