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Rivka M Lim^1,2, Thomas M Arme², Moses Arinaitwe^2,3

¹The University of Edinburgh Institute of Evolutionary Biology, Edinburgh, Scotland, UK.

Wellcome open research

|June 11, 2025

概括

由于幼虫阶段的DNA产量较低,Schistosoma mansoni的全基因组测序具有挑战性. 适应性采样没有足够丰富schistosomaDNA,突出显示样品洗对纯度的持续重要性.

科学领域:

基因组学就是基因组学.
寄生虫学的寄生虫学
分子生物学分子生物学

背景情况:

整个基因组测序 (WGS) 对于schistosoma基因组学至关重要.
成人虫无法进入,因此需要提取幼虫阶段的DNA.
幼虫样本 (miracidia) 的DNA含量很低,污染量很高,尤其是便.

研究的目的:

评估Nanopore的适应性采样,以丰富从幼虫阶段的Schistosoma mansoniDNA.
确定自适应采样是否可以从非侵入性收集的样本中提供足够的WGSDNA.

主要方法:

在洗过的和未洗过的Schistosoma miracidia上利用了纳米孔适应性采样.
雇佣了克拉肯2进行污染评估.
分析了对基因组覆盖的测序宽度和深度.

主要成果:

经过洗的样本显示了较高的Schistosoma mansoni DNA比例,证实了洗的有效性.
仅通过适应性抽样,就无法获得足够的Schistosoma mansoni对WGS的读数.
通过洗减少了污染,但适应性采样无法弥补低DNA输入.

结论:

洗对于最大限度地提高 Schistosoma mansoni DNA 纯度至关重要.
适应性采样目前还不足以为WGS丰富幼虫Schistosoma样本的DNA.
需要新的丰富策略来提高Schistosoma WGS.的测序效率和数据质量.

关键词:

适应性抽样采集方式奇迹般的奇迹的奇迹这是一个纳米孔.这种疾病叫做schistosomiasis.

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