流感诱导的microRNA-155表达在来自COPD表皮的细胞外囊中发生变化
Laura V Reid1, C Mirella Spalluto1,2, Tom M A Wilkinson1,2
1Clinical & Experimental Sciences, University of Southampton Faculty of Medicine, Southampton, United Kingdom.
Frontiers in cellular and infection microbiology
|June 11, 2025
概括
流感病毒感染改变了呼吸道细胞释放的细胞外囊泡 (EVs) 中的microRNA (miRNA) 水平. 在慢性阻塞性肺病 (COPD) 患者中,特定的miRNA (miR-155) 在EV中减少,可能会恶化流感的结果.
科学领域:
- 呼吸系统医学 呼吸系统医学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 流感病毒不成比例地影响患有慢性阻塞性肺病 (COPD) 的个体.
- 呼吸道上皮细胞是对流感的最初防御,释放含有microRNA (miRNA) 的细胞外囊泡 (EV).
- EV介导的miRNA转移可以调节对病毒感染的免疫反应.
研究的目的:
- 在流感感染时由支气管上皮细胞释放的EV微RNA进行分析.
- 在COPD患者中研究EV miRNA表达的变化.
主要方法:
- 在空气-液体界面 (ALI) 培养的支气管上皮细胞的流感感染.
- 通过过和大小排除色谱,将EVs从细胞培养超浮体中分离出来.
- 使用miRBase对EV miRNA货物的测序和分析.
- 使用RT-qPCR在健康和COPD个体的初级支气管上皮细胞中验证发现.
主要成果:
- 流感感染在支气管上皮细胞中的抗病毒基因上调,没有显著的细胞死亡.
- 差异表达分析揭示了五个关键的miRNAs (miR-155-5p,miR-122-5p,miR-378a-3p,miR-7-5p,miR-146a-5p) 在EVs.
- 来自COPD患者的EV显示,与对照组相比,miR-155的表达显著下降.
结论:
- 从上皮细胞衍生的EV及其miRNA载荷对于调节肺部对流感的反应至关重要.
- 变化的EV miRNA表达,特别是COPD中的miR-155降低,可能导致流感加剧的疾病.
- 需要进一步的研究来阐明EV微RNA在健康和COPD中的流感反应中的功能作用.
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