相关实验视频

Updated: Jun 13, 2025

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Published on: November 24, 2015

信号中心在mESC聚合物中驱动了分支周期动态和血统承诺

A Rabeling^1,2, M Goolam^1,2

¹Department of Human Biology, Faculty of Health Sciences University of Cape Town Cape Town South Africa.

FASEB bioAdvances

|June 11, 2025

概括

这项研究比较了基隆和BMP4信号如何影响小鼠胚胎干细胞聚合物. 信号中心,应用于聚合物的部分,更好地回顾了胚胎轴形成,而不是应用于整个聚合物的脉冲.

科学领域:

发展生物学发展生物学
干细胞生物学干细胞生物学
胚胎生成研究研究

背景情况:

基于干细胞的模型对于研究胚胎生成至关重要.
不同的协议使用不同的信号分子 (Chiron,BMP4) 和矩阵 (Matrigel).
这些组件和方法的具体作用尚未完全理解.

研究的目的:

为了比较基隆和BMP4信号的效果,将其应用为脉冲与小鼠胚胎干细胞聚合物的信号中心.
调查这些信号方法对形态学,Brachyury表达和血统承诺的影响.
阐明信号分子和应用方法对优化体外胚胎发育模型的贡献.

主要方法:

使用小鼠胚胎干细胞聚合模型.
应用基隆和BMP4信号作为脉冲 (整个聚合物) 或信号中心 (聚合物的部分).
嵌入式聚合物在低百分比的Matrigel中进行比较.

主要成果:

信号分子和应用方法 (脉冲与中心) 都对形态学,Brachyury表达和血统承诺产生了差异的影响.
作为脉冲,BMP4促进了神经分化.
信号中心更好地回顾了前后轴形成,显示了Brachyury极化和相关的基因表达.

结论:

与脉冲相比,信号中心提供了不同的发育线索.
BMP4和Chiron应用方法显著影响体外胚胎发育结果.
这些发现为基于干细胞的胚胎生成模型的实验设计提供了信息.

关键词:

没有信号通路.骨形态蛋白质 4 骨形态蛋白质 4 布拉基乌里蛋白质是一种蛋白质.胚胎和胎儿的发展.细胞外矩阵是细胞外矩阵.消化消化消化消化在现场混合化光光.神经发生神经发生.

更多相关视频

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Published on: March 19, 2021

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Published on: March 24, 2011

相关概念视频

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...

Cell Motility through Blebbing

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

Assembly of Signaling Complexes

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

Cytoskeletal Coordination in Cell Migration

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...