在朱中全基因组识别CDPKs和表达特征分析,以应对多种生物过程
Lili Li1, Ye Yuan1, Bingqi Shen1
1Institute of Fruits and Vegetables, Xinjiang Academy of Agricultural Sciences, Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Scientific Observation and Experimental Station for Pomology of Xinjiang, Ministry of Agriculture and Rural Affairs, Urumqi, Xinjiang, China.
Frontiers in plant science
|June 11, 2025
概括
这项研究确定了朱 (Ziziphus jujuba) 中21种依赖的蛋白激酶 (CDPK),分析了它们的基因结构,进化和表达. 这些ZjCDPK对朱果的生长和应激反应至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
背景情况:
- 依赖蛋白激酶 (CDPKs) 是植物生长,发育和应激反应的重要调节者.
- 关于朱 (Ziziphus jujuba Mill.) 中的CDPKs的信息有限. ) 是一个重要的水果作物.
研究的目的:
- 为了识别和描述朱中的CDPK基因家族 (ZjCDPKs).
- 分析 ZjCDPKs 的进化关系,基因结构和 cis 作用元素.
- 研究ZjCDPKs在各种生长阶段和压力条件下的表达模式.
主要方法:
- 全基因组识别和分析ZjCDPK基因.
- 用来自其他植物物种的CDPK进行了家族遗传学分析.
- 基因结构,保存动机和cis作用元素分析.
- 根据不同水果发育阶段和压力治疗 (植物质,冷,盐) 的表达特征.
主要成果:
- 确定了位于八个染色体上的21个ZjCDPK基因,其中大多数基因结构和基因图案都类似.
- 遗传学分析将ZjCDPK分为八个子组,揭示了与其他植物CDPK的进化关系.
- 分析确定了ZjCDPK促进体中的激素,压力,发育和光反应性cis作用元素.
- 大多数ZjCDPK在水果发育过程中以及对植物质感染,感冒和盐压力的反应中表现出差异性表达.
- 特定的ZjCDPKs (例如,ZjCDPK4/6/7/8/10/14/16) 显示与转录因子的相互作用和在压力下不同的表达模式.
结论:
- 这项研究提供了一个全面的分析ZjCDPK基因家族在jujube.
- 这些发现为ZjCDPKs在调节朱对发育线索和环境压力的反应方面的潜在作用提供了宝贵的见解.
- 这些结果为未来对朱中CDPKs的功能研究奠定了基础.
相关概念视频
02:34DNA Microarrays
17.2K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.2K
01:23Regulation of Expression at Multiple Steps
875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875
00:58Genomic DNA in Eukaryotes
46.8K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
46.8K
02:02Genomics
36.2K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.2K
01:58Cell Specific Gene Expression
13.5K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.5K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K