关于两种Penicillium chrysogenum菌株在豆发酵过程中的酶活性和代谢学的研究
Xiaodong Sun1, Hong Yang1, Guangwei Huo1
1College of Environment and Resources, Dalian Minzu University, Dalian, China.
Frontiers in microbiology
|June 11, 2025
概括
两个Penicillium chrysogenum菌株,JSPP6_1和GJY1_3,在发酵大豆粉生产中发挥着不同的作用. 菌株JSPP6_1增强了脂酶和有机酸水平,而GJY1_3增加了蛋白酶和氨基酸含量,影响了味道.
科学领域:
- 食品微生物学 食品微生物学
- 生物技术是生物技术.
- 代谢学 代谢学 代谢学
背景情况:
- 自然发酵的大豆依赖于微生物发酵来发展味道和质地.
- 了解微生物菌株的具体贡献对于优化发酵过程至关重要.
- Penicillium chrysogenum是一种已知的真菌,参与各种发酵过程.
研究的目的:
- 为了研究在豆发酵过程中两种Penicillium chrysogenum菌株 (JSPP6_1和GJY1_3) 之间的功能差异.
- 分析这些菌株的酶活性 (蛋白酶和脂酶) 和代谢概况.
- 确定每个菌株对影响大豆质量的关键代谢物的影响.
主要方法:
- 隔离和培养Penicillium chrysogenum菌株JSPP6_1和GJY1_3.3. 这两种菌株.
- 用单个菌株在45天内发酵大豆糊,定期采样.
- 在不同发酵阶段对蛋白酶和脂酶活动的测定.
- 在第15天对发酵样本进行代谢分析,以确定代谢物和代谢途径.
主要成果:
- 蛋白酶活性在GJY1_3 (108.95 U/mL) 和JSPP6_1 (70.79 U/mL) 的第15天达到峰值.
- 脂酶活性在JSPP6_1 (14.52 U/mL) 的第15天和GJY1_3 (7.41 U/mL) 的第20天达到峰值.
- 代谢分析显示了不同的特征:JSPP6_1显示了更高的脂酶和有机酸,而GJY1_3显示了更高的蛋白质酶和氨基酸 (包括像谷氨酸这样的风味化合物).
结论:
- 在豆发酵过程中,Penicillium chrysogenum菌株JSPP6_1和GJY1_3具有不同的功能作用.
- 菌株JSPP6_1与更高的脂酶产生和有机酸积累有关.
- 菌株GJY1_3对蛋白酶活性和关键氨基酸的发展有更多的贡献,影响了风味特征.
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