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相关概念视频

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

297
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
297

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Updated: Jun 12, 2025

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使用混合拉曼和部分波谱显微镜探测胃肠道中的癌症现场.

Elena Kriukova1,2, Mikhail Mazurenka1,2, Sabrina Marcazzan1,2

  • 1Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health & School of Computation, Information and Technology, Technical University of Munich, Munich 81675, Germany.

Analytical chemistry
|June 11, 2025
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概括
此摘要是机器生成的。

现场癌变 (FC) 检测通过使用联合拉曼和部分波谱学 (RS-PWS) 得到了改进. 这种混合方法识别了癌前组织中的分子和形态变化,提高了早期癌症查能力.

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科学领域:

  • 生物光子学和光谱学
  • 癌症研究 癌症研究
  • 分子病理学分子病理学

背景情况:

  • 现场癌症 (FC) 描述了恶性瘤之前的前恶性组织变化,对于早期癌症检测至关重要.
  • 现有的查方法可能无法完全捕捉微妙的,空间分布的癌前变化.
  • 需要新的光学技术来检测瘤邻近的宏观正常组织中的分子和形态变化.

研究的目的:

  • 评估Raman光谱法 (RS) 和部分波光谱法 (PWS) 在检测野外癌症中的有效性.
  • 研究RS和PWS在胃食道和肠道瘤模型中识别癌前变化的单独和综合性能.
  • 为了将光学发现与瘤邻近组织中的基因表达模式相关联.

主要方法:

  • 利用混合RS-PWS显微镜从L2-IL1B和Villin-Cre,Apc小鼠模型中的宏观正常瘤邻近组织中获取分子和形态数据.
  • 在数据分析中使用了部分最小平方差分分析 (PLS-DA).
  • 进行RNA测序,用于分析组织的转录基因分析.

主要成果:

  • 与对照组相比,RS检测到瘤携带小鼠正常组织中自由氨基酸带的显著增加和脂质和胡卜素带的减少.
  • 在L2-IL1B小鼠中,PWS数据提高了RS结果的分类准确性约9%,在Villin-Cre,Apc小鼠中提高了5%.
  • 转录组分析显示,观察到的光学变化和基因表达特征之间存在显著的相关性.

结论:

  • 结合RS-PWS有效地检测出在癌前组织中现场癌变的分子和形态变化.
  • 分子 (RS) 和结构 (PWS) 信息的协同使用提高了识别早期瘤变化的灵敏度.
  • 这种综合光谱方法为癌症发育过程中的组织变化提供了宝贵的见解,并有望改善癌症查.