表达特征和功能分析确定了新的累积细胞表达的基因和miRNAs,可以预测卵细胞发育潜力
Min Zhang1, Meng-Meng Wang1, Fa-Li Zhang1
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City 271018, China.
Cells
|June 11, 2025
概括
研究人员在累积细胞 (CCs) 中确定了与卵细胞发育潜力 (DP) 直接相关的特定基因和miRNAs. 这些发现为选择更有能力的卵细胞和胚胎提供了新的生物标志物,改善了试管婴儿的结果.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 累积细胞 (CCs) 表达影响卵细胞和胚胎质量的基因和miRNAs.
- 对于卵细胞发育潜力 (DP) 的现有标志物是有限的.
- 需要确定与DP直接相关的CC表达分子.
研究的目的:
- 识别和验证与卵细胞发育潜力 (DP) 相关的CC表达基因和miRNA.
- 建立可靠的生物标志物来选择合格的卵子和胚胎.
主要方法:
- 已建立的小鼠CC模型来自具有不同DP的累积卵细胞复合体 (COCs).
- 进行了mRNA/miRNA测序和功能分析.
- 利用井内井培养系统进行跟踪和验证.
主要成果:
- 鉴定了DP受益基因:Spp1,Fn1,Sdc1,Ngf.
- 鉴定了对DP有害的基因:Fos,Jun.
- 确定了对DP有害的miRNAs:miR-7686-5p,miR-133a-3p,新型miR-239,新型miR-193,miR-339-5p.
- 已验证的Spp1,Ngf,Jun,miR-7686-5p,新型miR-239和miR-339-5p与DP相关.
结论:
- 新的CC表达基因和miRNAs组与卵细胞DP直接相关.
- 这些分子显示出作为生物标志物选择有能力的卵细胞和胚胎的前景.
- 这些发现可能有助于增强辅助生殖技术.
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