先进的父亲年龄和精子蛋白质组动力学:与年龄相关的男性生育能力下降的可能解释
Joana Santiago1, Joana V Silva1, Manuel A S Santos2
1Department of Medical Sciences, Institute of Biomedicine, University of Aveiro, 3810-193 Aveiro, Portugal.
Cells
|June 11, 2025
概括
先进的父亲年龄 (APA) 影响男性生育能力. 蛋白质组分析揭示了精子蛋白和酸化的与年龄相关的变化,影响了生殖结果,并为生育能力评估提供了新的标记.
科学领域:
- 生殖生物学 生殖生物学
- 蛋白质组学是指蛋白质组学.
- 老龄化的分子机制.
背景情况:
- 男性生育能力随着父亲年龄的增长而下降 (APA).
- 与年龄相关的生育能力下降背后的分子机制尚不清楚.
- APA与降低生育能力,更差的生殖结果和后代健康问题有关.
研究的目的:
- 研究男性年龄对人类精子蛋白表达和酸化的影响.
- 识别精子中的分子变化,这可能解释与年龄相关的生育能力下降.
- 探索与年龄相关的男性不孕症的潜在生物标志物.
主要方法:
- 在四个年龄组 (≤30,31-35,36-40,>40岁) 中,对来自normozoospermic男性的人类精子样本进行蛋白质和光蛋白质分析.
- 质谱法用于评估蛋白质和酸化变化.
- 进行了基因本体学 (GO) 分析,以确定丰富的途径.
主要成果:
- 鉴定了46个差异表达蛋白 (DEP) 和94个差异化位 (对应于76个蛋白质).
- 老年男性的DEP与应激反应,新陈代谢和胚胎植入途径有关.
- 改变的酸盐与精子运动性,精子生成,精子区域光体结合以及代谢/应激反应途径 (例如,HSF1激活) 有关.
结论:
- 精子蛋白质和蛋白质随着男性年龄的增长而发生显著变化.
- 这些与年龄相关的精子分子变化与生育能力和生殖结果有关.
- 鉴定的分子标记物可以帮助评估生育能力,计划治疗,并解释与APA相关的异常不孕症或ART失败.
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