使用Sciex ZenoTOF 7600的非目标代谢学
Anna Artati1, Pauline Couacault1, Michael Witting2,3
1Metabolomics and Proteomics Core, Helmholtz Zentrum München GmbH, Neuherberg, Germany.
Methods in molecular biology (Clifton, N.J.)
|June 11, 2025
概括
非目标代谢学使用液体染色学高分辨率并联质谱法 (LC-HRMS/MS) 来分析各种代谢物. 结合逆相 (RP) 和水友交互液体色谱 (HILIC),可以提高生物样本中各种代谢类的覆盖率.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 非目标代谢学寻求全面的代谢物概况.
- 液体染色学与高分辨率并列质谱学 (LC-HRMS/MS) 结合,是主要的技术.
- 需要不同的染色学方法来覆盖广泛的代谢物极性.
研究的目的:
- 提出一个经过验证的非目标代谢学平台.
- 详细说明补充色谱技术的应用,以广泛覆盖代谢物.
- 为突出平台在各种生物矩阵的实用性.
主要方法:
- 使用液体染色学与高分辨率联质谱学 (LC-HRMS/MS) 相结合.
- 采用逆相 (RP) 和水友互动液体色谱 (HILIC) 进行分离.
- 验证了人类和动物生物液体和组织的平台.
主要成果:
- RP染色学有效地分析了半极到非极性代谢物 (例如脂肪酸,类固醇).
- 通过HILIC染色学,可以分析极性代谢物 (例如氨基酸,有机酸).
- 该平台在各种生物矩阵中展示了广泛的应用性.
结论:
- 结合RP和HILIC方法提供了广泛的代谢概况.
- 经过验证的LC-HRMS/MS平台适用于各种生物样本.
- 这种方法推进了生物系统中全面的代谢物分析.
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