对NAD+的代谢途径追踪合成和消费
Tumpa Dutta1, Stephen J Gardell2
1Translational Research Institute, AdventHealth, Orlando, FL, USA. tumpa.dutta@adventhealth.com.
Methods in molecular biology (Clifton, N.J.)
|June 11, 2025
概括
尼古丁胺胺氨基二核酸 (NAD+) 对新陈代谢和健康至关重要. 代谢途径追踪揭示了NAD+合成和消费如何影响水平,为衰老和疾病提供了洞察力.
科学领域:
- 生物化学 生物化学
- 代谢学 代谢学 代谢学
- 衰老研究研究 衰老研究
背景情况:
- 尼古丁胺胺氨基二核酸 (NAD+) 是一种重要的细胞代谢物,参与新陈代谢,氧化还原反应和细胞信号传输.
- 降低NAD+水平与衰老和慢性疾病有关,但根本原因 (合成与消费) 尚不清楚.
- 静态的NAD+测量不足以理解控制其水平的动态代谢途径.
研究的目的:
- 研究NAD+合成和消耗的动态过程.
- 阐明不同途径对稳定状态NAD+度的贡献.
- 批判性地评估NAD+代谢在衰老和疾病中的作用.
主要方法:
- 使用稳定同位素标记的NAD+前体 (例如尼古丁胺,尼古丁酸,三) 进行代谢途径追踪.
- 高分辨率质谱仪 (HRMS) 来追踪前体命运和代谢产物 (NADH,NADP,NADPH).
- 在各种生物样本 (细胞,组织,生物流体) 中分析NAD+合成和降解途径.
主要成果:
- 代谢途径的追踪成功地差异化了NAD+合成和消耗率.
- 该技术确定了NAD+前体和下游产品的代谢命运.
- 这种方法适用于从细胞培养到临床样本的各种生物系统.
结论:
- 代谢途径追踪是理解NAD+平衡的一个多功能工具.
- 它为NAD+代谢的动态调节提供了关键的见解.
- 这种技术可以帮助澄清NAD+水平,衰老和慢性疾病之间的联系.
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