可以在公牛精液冷保存过程中保护精子的功能属性
S Selvaraju1, N K S Gowda2, B Krishnappa3
1Animal Physiology Division, Reproductive Physiology Laboratory, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, 560030, India. Selvaraju.S@icar.org.in.
Biological trace element research
|June 11, 2025
概括
这项研究发现,将0.45μg添加到公牛精液扩展器中,在冷保存期间改善了精子运动性和线粒体膜潜力. 较高的剂量增加了活性氧物种和降低了精子功能.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 对哺乳动物生殖健康的影响尚不清楚.
- 冷保存公牛精液需要有效的延长剂来保持精子质量.
研究的目的:
- 调查在公牛精子冷保存期间,精液扩展器中补充剂的保护作用.
- 为了确定保护精子参数的最佳度.
主要方法:
- 泽西公牛的精液被收集并用基于Tris的扩展剂稀释,其中含有不同度的酸 (0,0.3,0.45,0.6μg/100μl).
- 精液样本被冷保存,解,并分析精子动力学,生命力,体完整性,膜潜力,ROS和染色质脱凝.
主要成果:
- 0.45μg/100μl显著改善了精子的总运动性和线粒体膜潜力 (MMP).
- 较高的剂量 (0.45和0.6μg/100μl) 增加了活性氧物种 (ROS) 的水平.
- 精子动力学和功能性膜完整性在最高的剂量 (0.6μg/100μl) 中显著下降.
- 补充剂增强了精子经历核染色体脱凝的能力.
结论:
- 精液扩展器中的0.45μg/100μl度有效地保持了公牛精子的运动性和冷后的MMP.
- 在较高的水平上增加的ROS可能表明线粒体活性增强,需要进一步调查.
- 现场生育研究是必要的,以证实作为精液扩展剂添加剂的潜力.
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