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转录组学的整合分析揭示了NETosis异质性和Ncf1作为中性喘中预后生物标志物

Qian Yan¹, Miaofen Zhang², Jing Yang²

¹First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Guangdong Provincial Clinical Research Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Postdoctoral Research Station of Guangzhou University of Chinese Medicine, Guangzhou 510405, China; State Key Laboratory of Traditional Chinese Medicine, Guangzhou 510405, China.

International immunopharmacology

|June 11, 2025

概括

中性粒细胞外细胞陷 (NETs) 驱动中性粒细胞性喘 (NA) 和葡萄糖皮质激素耐药性. NCF1被确定为NA的关键诊断生物标志物,提供潜在的治疗点.

科学领域:

免疫学免疫学免疫学
肺部病理学肺部病理学
生物标志物发现发现

背景情况:

中性粒细胞外细胞陷 (NETs) 涉及中性粒细胞性喘 (NA) 的发病.
在NA中对NETosis及其生物标志物的系统分析是有限的.

研究的目的:

在NA中识别NETs相关的生物标志物.
探索这些生物标志物的临床相关性和诊断潜力.

主要方法:

通过OVA/CFA敏感化建立了一种小鼠NA模型.
进行了转录基因分析 (RNA-seq) 和LASSO回归以识别与NETs相关的基因.
使用RT-qPCR,ROC分析和免疫光学验证的关键生物标志物.

主要成果:

在NA小鼠中,炎症增加,NETs组件 (Cit-H3/NE/MPO/PADI4),cfDNA和ROS.
NCF1被确定为一个关键的NETs生物标志物,对NA的诊断准确性很强.
NCF1表达与髓状细胞透相关,这表明它在NA发育中的作用.

结论:

由NCF1驱动的NETosis和髓状细胞相互作用是NA病原和葡萄皮质皮质体耐药性的关键.
NCF1作为NA的有价值的诊断生物标志物.
确定了NA干预的潜在治疗目标.

关键词:

拉索回归法 (Lasso Regression) 是一种回归法.巨细胞是一个巨细胞.中性粒细胞喘中性粒细胞外细胞陷通过RNA-seq技术的技术.

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