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Updated: Jun 13, 2025

08:51
Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
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界面/关闭:新型表面活性剂和蛋白质在固体-液体和油-液体界面上的竞争性吸附的表征
Vera Peters1, Leonie Schneider1, Sebastian Brosig1
1Novartis Pharma AG, TRD Biologics & CGT, Basel 4002, Switzerland.
Colloids and surfaces. B, Biointerfaces
|June 11, 2025
概括
新型表面活性剂VEDS和VEDG-3.3通过形成比传统选择更密集的界面层来改善蛋白质稳定性. 这项研究研究了它们在各种接口上与生物治疗药物吸附的行为.
科学领域:
- 生物制药配方科学 生物制药配方科学
- 表面化学 表面化学
- 蛋白质稳定 蛋白质稳定
背景情况:
- 生物制药面临界面压力,需要像表面活性剂这样的保护辅助剂.
- 已建立的表面活性剂 (多酸盐,波洛克萨默188) 和它们在空气液体接口上的机制已得到充分研究.
- 关于新型表面活性剂及其在固体-液体和油-液体界面上的行为,与各种生物治疗药物存在有限的研究.
研究的目的:
- 研究新型表面活性剂 (VEDS,VEDG-3.3) 和已确定的表面活性剂在固体-液体和油-液体接口的吸附行为.
- 评估三种模型生物治疗药物对表面活性剂吸附的影响.
- 阐明新型表面活性剂用于增强蛋白质稳定性的界面机制.
主要方法:
- 石英晶体微平衡与消耗 (QCM-D) 监测.
- 张力计用于界面张力测量.
- 吸附研究涉及模型生物治疗和表面活性剂在不同的接口.
主要成果:
- 表面活性剂的吸附依赖于分子,受蛋白质存在和接口类型的影响.
- 在同时或顺序添加的情况下,观察到蛋白质和表面活性剂的协同吸附.
- 与已建立的表面活性剂相比,VEDS和VEDG-3.3的吸附动力学和随后的分子重新排列速度较慢,导致更密集的界面包装.
结论:
- 新型表面活性剂VEDS和VEDG-3.3显示出不同的吸附行为,有利于蛋白质稳定.
- 这些发现支持在药物相关接口上的蛋白质-表面活性剂混合物的统一热力学模型.
- 这项研究为VEDS和VEDG-3.3在生物制药配方中的优越性能提供了机械洞察力.
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