相关实验视频
Updated: Jun 13, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
通过miR-200过度表达或Crispr-Cas介导的淘汰赛减少JAG1表达会影响TNBC生长和转移
Megan Vaz1, Katrina L Watson1, Roger A Moorehead1
1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
在三阴性乳腺癌 (TNBC) 中增加miR-200表达,通过降低JAG1来减少瘤生长. 针对JAG1可能为TNBC患者提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 增加三阴性乳腺癌 (TNBC) 的miR-200表达以前已经被证明可以减少瘤生长和转移.
- 在TNBC中,miR-200发挥其瘤抑制作用的精确分子机制需要进一步阐明.
研究的目的:
- 调查JAG1作为TNBC中miR-200的下游目标的作用.
- 在TNBC模型中评估针对JAG1的治疗潜力.
主要方法:
- 在TNBC细胞系中,miR-200s的过度表达.
- 在MDA-MB-231细胞中JAG1基因被淘汰.
- 在体外扩散和入侵测定.
- 在小鼠模型中的体内瘤生长和转移研究.
- 对RNA测序的分析.
主要成果:
- 在TNBC细胞中miR-200s的过度表达导致JAG1表达的显著减少.
- 在MDA-MB-231细胞中JAG1的淘汰在体外显著降低了细胞的增殖和侵入.
- 失去JAG1抑制了乳腺瘤的生长和体内转移.
- RNA测序显示,JAG1损失改变了参与细胞外基因组 (ECM) 改造,血管生成和上皮-介质细胞过渡 (EMT) 的基因.
结论:
- miR-200可能通过降低JAG1表达的调节,在TNBC中发挥其抗瘤作用.
- 抑制JAG1证明了TNBC的治疗潜力.
- 准JAG1值得进一步研究,作为治疗三阴性乳腺癌的治疗策略.
更多相关视频
10:32Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
Published on: December 19, 2019
07:41A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
Published on: March 8, 2022
相关概念视频
MicroRNAs
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Mitogens and the Cell Cycle