相关实验视频

Updated: Jun 13, 2025

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

基于脂质组的RNA传递系统提高了RNAi的效率,用于向控制虫在Spodoptera litura中的虫

Yun-Heng Lu¹, Yun Liu¹, Yu-Chun Lin¹

¹Department of Entomology, National Taiwan University, Taipei 106, Taiwan.

International journal of biological macromolecules

|June 11, 2025

概括

立方体纳米颗粒为RNA干扰 (RNAi) 害虫控制提供了稳定有效的传递系统. 这种新的方法增强了昆虫的RNA保护和基因沉默,显示了可持续农业的前景.

科学领域:

生物技术是生物技术.
纳米技术纳米技术
昆虫学昆虫学是一门学科.

背景情况:

RNA干扰 (RNAi) 是通过沉默重要昆虫基因来实现可持续害虫控制的一个有希望的策略.
由于环境的不稳定性和RNA分子的低效传递,RNAi的实际应用受到阻碍.

研究的目的:

开发新的RNA传递纳米粒子,以提高RNA稳定性和基因沉默效率.
为了评估Polyplex,CS-TPP和Lipoplex纳米粒子与Spodoptera litura中的网状基因对比.

主要方法:

开发了三种新的RNA传递纳米粒子:Polyplex,CS-TPP和Lipoplex.
对抗热,紫外线辐射和RNA酶的RNA保护的评估.
在实验室中使用S. litura SL1A细胞系进行细胞吸收研究.
在体内生物测试和叶子生物测试以评估基因沉默和死亡率.

主要成果:

脂质检测证明了双链RNA (dsRNA) 和小干扰RNA (siRNA) 对降解的优越保护.
利波普莱克斯显著增强了S. litura细胞中的siRNA内部化.
装有siRNA的Lipoplex有效抑制了网状基因表达,损害了幼虫的发育,并导致S. litura幼虫的高死亡率.
叶子生物测试证实了Lipoplex在环境条件下的稳定性和有效性.

结论:

立方体纳米颗粒作为一种强大的纳米载体系统,用于基于RNAi的害虫防治.
利波普莱克斯既能提供保护,也能有效地在目标昆虫中传递RNA分子到细胞内.
这项技术对可持续和有效的昆虫害虫管理具有重大前景.

关键词:

生物杀虫剂生物杀虫剂这就是MESH的意义.纳米颗粒纳米颗粒这是一种RNA干扰.它们的形形虫 (Spodoptera litura) 已经被发现.

更多相关视频

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Published on: May 1, 2018

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...